1. Swiss – Indonesian Vaccine Formulation Symposium
EVALUATION OF THE PANDEMIC POTENTIAL OF H5N1 HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUSES CIRCULATING IN INDONESIA
Swiss – Indonesian
Vaccine Formulation Symposium

2. Background
Since late H5N1 subtype Avian Influenza vius had spread in poutry in several Asian countries include China, Vietnam, Thailand, Cambodia, Korea and Indonesia, also some countries in Europe and Africa ((WHO,2013)
In Indonesia, every year the viruses have caused out break among poultry, producing highest number of human victim worldwide, until October 15, 2015, Indonesia had more than 199 confirmed human cases with 167 deaths (WHO,2016)

3. Based on recent report that Bats (order Chiroptera) and pigs are of particular interest the source of multiple pathogenic viruses for human and domestic animal.
Isolation and identification of influenza viruses in bats and others animals in Indonesia is very necessary, considering that Indonesia is endemic regions againts influenza viruses, especially avian influenza virus

4. Method and Material
Samples : fruits bats and wild pigs collected from Kalimantan and Java Island
Sera were collected at the field and tissues specimens were obstained at Animal BSL3 Laboratory in Avian Influenza Research Center Univeritas Airlangga at January 2013 –April 2015.
All sera and tissue samples stored -80 oC until analysed.
Samples inoculated to eggs and cell culture, HA test, HA primes of PCR

5. Result
These research were total of 216 animals had been collected from Kalimantan and Java island were 46 of 216 samples positive of HA test, 17 of 216 positive by antisera H5 test and 4 of 216 samples positive by PCR test

6. Data of Bats

7. Antigenic Finding Serological Finding
Avian Influenza-zoonoses Research Center

8. HI test identification subtipe H5 HI test identification subtipe H5
Samples code
HA test
(titer HA)
HI test identification subtipe H5 25 23 + 26 - 27 29 28 30 31 32 33 34 21 35
Samples code
HA test
(titer HA)
HI test identification subtipe H5 36 - 37 21 38 39 40 41 42 43 44 45 46 47 48

9. Tabel 5.1 Hasil Positif Uji HA Pasase Kedua pada TABNo
Kode Lapangan
Hasil Uji HA
(Titer HA) 1 A 25
GR 1.18 2 A 26
GR 1.19 24 3
A.6.6 4 27
Ng 1.16 4 A 28
Ng 1.19 5
Kaltim 12A 29
Ng 1.28 6
Kaltim 24A 30
BWI 1 7
Kaltim 79 1 31
BWI 6 8
Kaltim 79 3 32
BWI 9 9
Kaltim 79 5 33
BWI 27 10
Kaltim 79 6 34
BWI 32 11
Kaltim 79 7 35
BWI 33 12
Kaltim 79 9 36
Blora 2 Lok 1 Kel 2 13
PKY 2 37
Blora 2 Kel 1 14
KK 10 38
Blora 2 Kel 2 15
KK 11 39
JT 1.2 16
KK 13 40
JT 1.4 17
KK 15 41
JT 1.5 18
KK 17 21 42
JT 1.6 19
KK 21 43
JT 1.7 20
KK 23 44
Jember 2
KK 30 45 A 22
GR 1.6 46 A 23
GR 1.12 47 A
GR 1.17 48
A.6.6 Plastik

10. Tabel 5.2 Hasil Negatif Uji HA Pasase Ketiga Pada TAB No
Kode Lapangan
Hasil Uji HA (Titer HA) 1 A - 25
GR 1.18 2 A 26
GR 1.19 3
A.6.6 4 27
Ng 1.16 4 A 28
Ng 1.19 5
Kaltim 12A 29
Ng 1.28 6
Kaltim 24A 30
BWI 1 7
Kaltim 79 1 31
BWI 6 8
Kaltim 79 3 32
BWI 9 9
Kaltim 79 5 33
BWI 27 10
Kaltim 79 6 34
BWI 32 11
Kaltim 79 7 35
BWI 33 12
Kaltim 79 9 36
Blora 2 Lok 1 Kel 2 13
PKY 2 37
Blora 2 Kel 1 14
KK 10 38
Blora 2 Kel 2 15
KK 11 39
JT 1.2 16
KK 13 40
JT 1.4 17
KK 15 41
JT 1.5 18
KK 17 42
JT 1.6 19
KK 21 43
JT 1.7 20
KK 23 44
Jember 2 21
KK 30 45 A 22
GR 1.6 46 A 23
GR 1.12 47 A 24
GR 1.17 48
A.6.6 Plastik

11. M K K+

12. M K K+
1780 bp

13. Tabel 5.3 Hasil Realtime Menggunakan Primer Gen Matrix
Sample Name Ct
Qty
GR-1.17
6.46
PKY-2
Undet.
KK-17
GR-1.19
DM-78
15.66
349.88
D41
14.25
450.32

14. Tabel 5.4 Hasil Realtime Menggunakan Primer Gen H5
Sample Name Ct
Qty
GR1.17
30.25
7.08
D4(1)
14.45
929.19
D4(2)
14.77
840.75
DM78
16.05
566.46

19. Conclusion Bats may be infected Influenza
Wild pigs may be infected influenza
Surveilance is necessary to describe epidimiology of disease

20. Thank You

21. Source : http://www.downtoearth.org
Bats-Borne Disease
Source :
Avian Influenza-zoonoses Research Center