1. Contribution of nitric oxide to the pathogenesis of cirrhotic cardiomyopathy in bile duct– ligated rats 
Hongqun Liu, Zenghua Ma, Samuel S. Lee 
Gastroenterology 
Volume 118, Issue 5, Pages (May 2000)
DOI: /S (00)
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2. Fig. 1
RT-PCR of NOS2 transcription from rat hearts. Lane 1, 1-kilobase (kb) ladder; lanes 2 and 3, control; lanes 4 and 5, BDL; lane 6, lipopolysaccharide (LPS)-treated positive control. Computerized optical densitometry indicated a significant difference between controls (n = 6) and BDL (n = 6) (P < 0.01).
Gastroenterology  , DOI: ( /S (00) )
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3. Fig. 2
Western blot analysis of NOS2 protein expression in hearts. Lane 1, lipolysaccharide endotoxin treatment as a positive control; lanes 2 and 3, sham-operated controls; lanes 3 and 4, BDL. Computerized optical densitometry showed a significant difference between controls (n = 6) and BDL (n = 6) (P < 0.05).
Gastroenterology  , DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Imunohistochemical staining for NOS2. No immunoprecipitate is observable on the slide from the sham control. In the cirrhotic heart, NOS2-positive cells are localized in cardiomyocytes (original magnification 50×).
Gastroenterology  , DOI: ( /S (00) )
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5. Fig. 4
RT-PCR of NOS3 transcription from rat hearts. Lane 1, 1-kb ladder; lanes 2 and 3, control; lanes 4 and 5, BDL. Computerized optical densitometry indicated no significant difference between controls (n = 6) and BDL (n = 6).
Gastroenterology  , DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

6. Fig. 5
Western blot analysis of NOS3 protein expression in hearts. Lane 1, NOS3 from human endothelium as a positive control; lanes 2 and 3, sham-operated controls; lanes 4 and 5, BDL. Computerized optical densitometry showed no significant difference between controls (n = 6) and BDL (n = 6). kDa, kilodaltons.
Gastroenterology  , DOI: ( /S (00) )
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7. Fig. 6
Cumulative dose-responses to isoproterenol in isolated left ventricular papillary muscles from BDL and sham-operated rats (n = 6 for each of the 4 groups). Maximal response (Rmax) for the BDL group was significantly lower than the others. No significant differences were observed in EC50 values among the 4 groups.
Gastroenterology  , DOI: ( /S (00) )
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8. Fig. 7
Effects of the exogenous NO donor SNAP on isoproterenol-stimulated maximal papillary muscle contractility. Contractility was significantly decreased in the SNAP-treated group (n = 6 in each group). *P < 0.05 compared with control.
Gastroenterology  , DOI: ( /S (00) )
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9. Fig. 8
Effects of IL-1β and L-NAME on isoproterenol-stimulated maximal left ventricular papillary muscle contractility. Decreased contractile force with IL-1β was reversed by L-NAME coincubation (n = 6 in each group). *P < 0.05 compared with control and IL-1β+L-NAME group.
Gastroenterology  , DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions