1. Que. 1 Which of the following methods will not sterilize?
A) 500 mg/L ethylene oxide at 50 C for 4 hours
B) aqueous glutaraldehyde for 10 hours
C) dry heat at 171 C for 1 to 2 hours
D)121 C at 15 lb/in pressure for 15 to 20 minutes
E)100  C boiling water for 30 minutes

2. Identify the correct definition of an antiseptic (रोगाणुरोधक):
Que. 2
Identify the correct definition of an antiseptic (रोगाणुरोधक):
A. Chemicals that kill or prevent infection and damage living tissues
B. Chemicals that kill or prevent infection without damaging living tissues
C. Chemicals that can only kill or prevent infection on non-living tissues
D. Chemicals that can only kill or prevent infection on animal housing

3. Identify the correct definition of a disinfectant:
Que. 3
Identify the correct definition of a disinfectant:
A. Chemicals that kill or prevent infection and damage living tissues.
B. Chemicals that kill or prevent infection without damaging living tissues.

4. Que. 4
Identify the term that is used to ensure surgical instruments are free from micro-organisms:
A. Disinfected
B. Cleaned
C. Debrided
D. Sterilization

5. Que. 5
Identify the term that can describe a disinfectant that can inhibit the growth of fungi.
Fungicidal
Microbicidal
fungistatic
microbiostatic

6. Which of the following is a limitation of
Que. 6
Which of the following is a limitation of
the autoclave?
A. It lacks the ability to kill endospores
B. It cannot be used with glassware
C. It lacks the ability to inactivate viruses
D. It will destroy heat labile materials
E. It takes too long to sterilize

7. Que. 7 Each of the following statements concerning the
killing of bacteria is correct EXCEPT:
A. The pasteurization of milk kills pathogens but allows
many organisms and spores to survive.
B. A 70% solution of ethanol kills more effectively than
absolute (100%) ethanol.
C. An autoclave uses steam under pressure to reach the
killing temperature of 121°C.
D. Iodine kills by causing the formation of thymine
dimers in bacterial DNA.

8. The efficiency of moist-heat sterilization is tested
Que. 8
The efficiency of moist-heat sterilization is tested
with
A. Vegetative cells of Bacillus stearothermophilus
B. Clostridium botulinum
C. Spores of aerobic bacteria
D. Spores of Bacillus stearothermophilus

9. Que. 9
Which of the following is most effective for sterilizing mattresses and plastic Petri plates?
Ethylene oxide
Autoclave
Ultraviolet radiation
Chlorine
Glutaraldehyde

10. Que. 10
Which of the following method is best to sterilize heat labile solutions?
 Autoclave
 Pasteurization
 Hot Air Oven
 Membrane filtration

11. Que. 11 Lyophilazation means Sterilization Freeze Drying
 Burning to ashes
 Exposure to heat.

12. Temperature used for hot air oven
Que. 12
Temperature used for hot air oven
100 C for 1min
80 C for 10 min
160 C for 2 hrs
160 C for 2 min.

13. Glassware are sterilized by?
Que. 13
Glassware are sterilized by?
Hot air oven
Autoclaving
Incineration
None of these

14. Which method is not used for obtaining pure culture of bacteria?
Que. 14
Which method is not used for obtaining pure culture of bacteria?
Streak plate method
Pour plate method
Transformation method
Spread plate method.

15. Agar is obtained from? Que. 15 Red Algae Green Algae Purple Bacteria
Green Bacteria

16. Que. 16
______________ is needed as a source of nutrient for the growth and reproduction of microbes.
pathogens
reagents
bacteria
media

17. Method used for maintenance and preservation of culture?
Que. 17
Method used for maintenance and preservation of culture?
Lyophilization
Sub Culturing
Overlaying with oil
All of the above.

18. Que. 18
An   is a medium with specific and known qualities that favors the growth of a particular microorganism.
enrichment culture
Selective medium
Differential medium
None of the above

19. Nutrient broth is the example of
Que. 19
Nutrient broth is the example of
Selective media
Differential media
Basal media
None of the above

20. Cryopreservation is Que. 20 A: preservation at low temperature
B: preservation at high temperature
C: preservation with dehydration
D: preservation in mineral oil

21. Que. 21
Which of the following method used for the preservation of fungus/ Spores?
A: Periodic transfer to fresh media
B: Storage in sterile Sand/Soil
C: Storage in normal saline
D: Overlaying culture with mineral oil

22. A group of individual cells that share a common ancestry is ?
Que. 22
A group of individual cells that share a common ancestry is ?
Strain
Isolate
Clone
None of the above

23. Radiant Quadrant T streak Continuous Que. 23
Identify the streaking method of
given figure
Radiant
Quadrant
T streak
Continuous

24. Answer Key
1.E
2.C
3.A
4.D
5.3
6.D
7.D
8.D
9.A
10.D
11.B
12. C
13. A
14. C
15. A
16. D
17. D
18. A
19.C
20. A
21. B
22. C
23. B

25. Que 2& 3
An antiseptic is used on living tissues, cells and non living tissues to destroy any types of infections which may be living on the tissue. Disinfectants are meant to destroy microorganisms which can infect nonliving objects. Common antiseptics include mouthwash, and yeast infection treatment creams.

26. Que 5
The simplest, most stringent definitions identify fungistatic drugs as those that inhibit growth, whereas fungicidal drugs kill fungal pathogens.

27. Que 7
Iodine damages the walls of the cells that make up the micro-organisms and causes the insides to leak out of the cells. This stops the micro-organisms from being able to make important proteins that are necessary for the organism to survive, so the micro-organism dies, and the infection disappears.

28. Que 15
The gelling agent in agar is an unbranched polysaccharideobtained from the cell walls of some species of red algae, primarily from the genera Gelidium and Gracilaria. For commercial purposes, it is derived primarily from Gelidium amansii. In chemical terms, agar is a polymer made up of subunits of the sugar galactose.

29. Que 18
An enrichment culture is a medium with specific and known qualities that favors the growth of a particular microorganism. The enrichment culture's environment will support the growth of a selected microorganism, while inhibiting the growth of others.

30. Que 20
Cryopreservation or cryoconservation is a process where organelles, cells, tissues, extracellular matrix, organs or any other biological constructs susceptible to damage caused by unregulated chemical kinetics are preserved by cooling to very low temperatures (typically -80 °C or -196 °C) 

31. Que 22
CLONE: an organism or cell, or group of organisms or cells, produced asexually from one ancestor or stock, to which they are genetically identical.