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Fig. 6. Effect of Constitutively Active MEKK on JNK Activity αT3–1 cells were cotransfected with either N-terminally deleted, constitutively active MEKK1.

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Presentation on theme: "Fig. 6. Effect of Constitutively Active MEKK on JNK Activity αT3–1 cells were cotransfected with either N-terminally deleted, constitutively active MEKK1."— Presentation transcript:

1 Fig. 6. Effect of Constitutively Active MEKK on JNK Activity αT3–1 cells were cotransfected with either N-terminally deleted, constitutively active MEKK1 (MEKK), or vector control together with HA-JNK2 as described in Materials and Methods. Two days after transfections the cells were serum starved for 16 h followed by a treatment with GF109203X (GF, 3 μm, 15 min), genistein (Gen, 200 μm, 15 min), GnRH-a (10<sup>−7</sup>m, 30 min), or control PBS as indicated. GF109203X and genistein had no effect even when added for up to 2 h. Comparable amounts of JNK were detected in the immunoprecipitants of all lanes (data not shown). From: Stimulation of Jun N-Terminal Kinase (JNK) by Gonadotropin-Releasing Hormone in Pituitary αT3–1 Cell Line Is Mediated by Protein Kinase C, c-Src, and CDC42 Mol Endocrinol. 1998;12(6): doi: /mend Mol Endocrinol | Copyright © 1998 by The Endocrine Society 1

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