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Developmental validation of the PowerPlex® Fusion System for analysis of casework and reference samples: A 24-locus multiplex for new database standards 

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Presentation on theme: "Developmental validation of the PowerPlex® Fusion System for analysis of casework and reference samples: A 24-locus multiplex for new database standards "— Presentation transcript:

1 Developmental validation of the PowerPlex® Fusion System for analysis of casework and reference samples: A 24-locus multiplex for new database standards  Kathryn Oostdik, Kristy Lenz, Jeffrey Nye, Kristin Schelling, Donald Yet, Scott Bruski, Joshua Strong, Clint Buchanan, Joel Sutton, Jessica Linner, Nicole Frazier, Hays Young, Learden Matthies, Amber Sage, Jeff Hahn, Regina Wells, Natasha Williams, Monica Price, Jody Koehler, Melisa Staples, Katie L. Swango, Carolyn Hill, Karen Oyerly, Wendy Duke, Lesley Katzilierakis, Martin G. Ensenberger, Jeanne M. Bourdeau, Cynthia J. Sprecher, Benjamin Krenke, Douglas R. Storts  Forensic Science International: Genetics  Volume 12, Pages (September 2014) DOI: /j.fsigen Copyright © 2014 The Authors Terms and Conditions

2 Fig. 1 Electropherograms showing detectable peaks with the indicated species using the PowerPlex® Fusion System. Peaks were detected with 10ng of chicken, mouse, or pig DNA. Samples were detected using an Applied Biosystems® 3130xl Genetic Analyzer with a 3kV 5s injection. Peak amplitude threshold was 50 RFU (n=2). Forensic Science International: Genetics  , 69-76DOI: ( /j.fsigen ) Copyright © 2014 The Authors Terms and Conditions

3 Fig. 2 Percent alleles called (left-hand axis; light gray) and average sample peak height ratio (right-hand axis; dark gray) over a DNA titration from 500pg to 50pg. Samples were detected using an Applied Biosystems® 3130 and 3500 Series Genetic Analyzers using 3kV 5s or 1.2kV 10s injections, respectively, and a 3730 DNA Analyzer using a 3kV 5s injection. Error bars represent standard deviation (n=36). Forensic Science International: Genetics  , 69-76DOI: ( /j.fsigen ) Copyright © 2014 The Authors Terms and Conditions

4 Fig. 3 Electropherograms showing D12S391 allele 18.3 with the allele 19 shoulder. Samples were detected using the Applied Biosystems® 3500xl and the 3130xl Genetic Analyzers with a 1.2kV 24s or 3kV 5s injection, respectively. Similar resolution was observed on the Applied Biosystems® 3730 DNA Analyzer. Forensic Science International: Genetics  , 69-76DOI: ( /j.fsigen ) Copyright © 2014 The Authors Terms and Conditions

5 Fig. 4 Size standard deviation for each allele within the PowerPlex® Fusion Allelic Ladder Mix. One microliter of allelic ladder was detected using a Applied Biosystems® 3500xl Genetic Analyzer at Promega Corporation using a 1.2kV 24s injection. Similar results were obtained at two other sites (n=48). Forensic Science International: Genetics  , 69-76DOI: ( /j.fsigen ) Copyright © 2014 The Authors Terms and Conditions

6 Fig. 5 Percent alleles called using extracted DNA or pretreated solid support materials with reduced-volume reactions. Samples were detected using Applied Biosystems® 3130xl Genetic Analyzers with a 3kV 5s injection. Error bars represent standard deviation. (Extracted DNA, n=3; Swab lysate, n=4; FTA® card punch, n=27.) Forensic Science International: Genetics  , 69-76DOI: ( /j.fsigen ) Copyright © 2014 The Authors Terms and Conditions

7 Fig. 6 Percent alleles called using nonFTA punches as the template source in reduced-volume reactions. Reactions were amplified in the presence and absence of AmpSolution™ Reagent (n=6). Forensic Science International: Genetics  , 69-76DOI: ( /j.fsigen ) Copyright © 2014 The Authors Terms and Conditions

8 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

9 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

10 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

11 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

12 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

13 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

14 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

15 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

16 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

17 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

18 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

19 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

20 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions

21 Forensic Science International: Genetics 2014 12, 69-76DOI: (10.1016/j.fsigen.2014.04.013)
Copyright © 2014 The Authors Terms and Conditions


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