1. Neither normal nor diseased placentas contain lymphatic vessels
E. Castro, W. Tony Parks, C. Galambos 
Placenta 
Volume 32, Issue 4, Pages (April 2011)
DOI: /j.placenta
Copyright © 2011 Elsevier Ltd Terms and Conditions

2. Fig. 1
Immunohistochemical localization of the vascular components of first trimester (8 weeks) and early third trimester (30 weeks) placentas. A and B: H&E staining. (A) First trimester placenta and (B) early third trimester placenta (original magnification 400X). C and D: CD31 immunostaining. (C) First trimester placenta and (D) early third trimester placenta. Membranous brown staining identifies endothelial cells (original magnification 400X). E and F: PROX-1 immunostaining. (E) First trimester placenta and (F) early third trimester placenta (original magnification 400X). Brown staining is non-specific; no nuclear-positive lymphatic vascular cells are identified (arrow). G and H: D2-40 immunostaining. (G) First trimester placenta and (H) early third trimester placenta (original magnification 400X). Brown staining highlights the stromal meshwork; endothelial cells are negative (arrow).
Placenta  , DOI: ( /j.placenta )
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3. Fig. 2
Immunohistochemical localization of the vascular components of a term placenta. A and B: H&E staining of the placental basal plate (original magnification 200X (A) and 400X (B)). C and D: CD31 immunostaining of the placental basal plate. Brown staining identifies endothelial cells (original magnification 200X (C) and 400X (D)). E and F: D2-40 immunostaining of the placental basal plate. Brown staining identifies lymphatic vessels (original magnification 200X (E) and 400X (F)). G and H: PROX-1 immunostaining of the placental basal plate. Nuclear brown staining identifies lymphatic vessels (original magnification 200X (G) and 400X (H)).
Placenta  , DOI: ( /j.placenta )
Copyright © 2011 Elsevier Ltd Terms and Conditions

4. Fig. 3
Immunohistochemical localization of the vascular components of a 24 week placenta. A: D2-40 immunostaining of the villous vessels. The mesenchymal cells in the chorionic villous core are positive for D2-40 (original magnification 400X); the endothelial lining cells are negative. B. CD31 immunostaining of the villous vessels (original magnification 400X). Brown staining identifies the endothelial lining. C: Reticulin stain of the chorionic villous core (original magnification 400X). The chorionic villous core mesenchymal cells show a pattern of staining similar to that seen with D2-40 (A). D: SMA immunostaining of the villous vessels (original magnification 400X). Brown stain identifies the pericytes surrounding the blood vessels; no stain is identified in the stromal mesenchymal cells.
Placenta  , DOI: ( /j.placenta )
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5. Fig. 4
Immunohistochemical localization of the vascular components of a placental mesenchymal dysplasia case. A: H&E staining of dilated cisterns (original magnification 100X). B: H&E staining of the cistern lining (arrow) (original magnification 200X). C: Reticulin staining of dilated cisterns (original magnification 400X). Reddish stain identifies the reticulin meshwork in the cistern wall. D: CD31 staining of dilated cisterns (original magnification 400X). No positive brown stain is identified in the cistern lining. E: D2-40 immunostaining of dilated cisterns (original magnification 400X). No positive brown staining is identified in the cistern lining (arrow). F: PROX-1 immunostaining of dilated cisterns (original magnification 400X). No brown nuclear staining is identified in the cistern lining.
Placenta  , DOI: ( /j.placenta )
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6. Fig. 5
Immunohistochemical localization of the vascular components of other edematous placental disorder cases. A and B: Placenta from a complete hydatiform mole. A. D2-40 immunostaining of the dilated villi (original magnification 200X). Brown staining is identified in the villous core mesenchymal cells surrounding the cistern lining. B. CD31 immunostaining of the dilated villi (original magnification 200X). No positive stain is seen in the lining. C and D: Placenta from a partial hydatiform mole. C: D2-40 immunostaining of the dilated villi (original magnification 200X). Brown stain is identified in the mesenchymal cells around the cistern. D: CD31 immunostaining of the dilated villi (original magnification 200X). Brown staining identifies remaining fetal vessels. E and F: Placenta from a hydropic abortion. E: D2-40 immunostaining of the dilated villi (original magnification 200X). Brown stain is identified in the mesenchymal cells within the edematous villi F: CD31 immunostaining of the edematous villi (original magnification 200X). No remaining fetal vessels are identified in the chorionic villi.
Placenta  , DOI: ( /j.placenta )
Copyright © 2011 Elsevier Ltd Terms and Conditions