1. Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
Altogen Biosystems offers the HUH-7 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
The HUH-7 Transfection Reagent is an advanced biodegradable polymer based transfection reagent that enhances lipid mediated transfection, and it has been developed to provide high transfection efficiency with HUH-7 cells.
This cell line is a good host for studying liver cancer. When cultured in vitro, HUH-7 cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase HUH-7 Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
The HUH-7 cell line originates from the liver tumor of a 57-year old male Japanese patient. The cell line was established in 1982 by Nakabayshi, H. and Sato, J. The HUH-7 cell line is a well differentiated cellular carcinoma cell line that is a type of immortal cell line. As the cell line is a hepatoma, the cells display epithelial cell morphology. Liver cancer can occur commonly as a result of cirrhosis (alcohol-induced) or viral hepatitis infection.
HUH-7 Cells
cellbank.nibiohn.go.jp
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. HUH-7 Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
HUH-7 Transfection Protocol
1. Plate 10, ,000 HUH-7 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with HUH-7 cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of HUH-7 cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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5. HUH-7 Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
HUH-7 Transfection Kit Product Details
A biodegradable polymer based transfection reagent – once inside the cell, the polymer degrades into smaller less toxic components reducing cell toxicity, facilitating release of the transgene, and improving transfection efficiency
Transfection protocols provided for transfection of proteins, DNA, mRNA, siRNA, shRNA and microRNA
Transfection Enhancer reagent provided with the kit
Produce higher level of recombinant protein expression with minimal disruption of normal cell function
Generate physiologically relevant data you can trust
Effective for plasmid DNA/siRNA co-transfection
Easy-to-use transfection protocol with reproducible results
Low cytotoxicity
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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6. Data Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
Data
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the Huh-7 cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPD mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=5).

7. Data Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
Data
Figure 2. Protein expression of GAPDH in HUH-7 cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into HUH-7 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. HUH-7 Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > HUH-7 Transfection Reagent (Liver Cancer Cells)
HUH-7 Transfection Kit Benefits
Pre-optimized transfection protocol for HUH-7 cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use HUH-7 transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable HUH-7 cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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