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Wen Chin Chiang, MRCPCH, Chiung-Hui Huang, PhD, Genevieve V

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Presentation on theme: "Wen Chin Chiang, MRCPCH, Chiung-Hui Huang, PhD, Genevieve V"— Presentation transcript:

1 Anaphylaxis to cow's milk formula containing short-chain galacto-oligosaccharide 
Wen Chin Chiang, MRCPCH, Chiung-Hui Huang, PhD, Genevieve V. Llanora, MD, Irvin Gerez, MD, Si Hui Goh, MBBS, Lynette P.C. Shek, MRCP, Alma J. Nauta, PhD, Wilhelmina A. Van Doorn, BSc, Jacques Bindels, PhD, Laurien H. Ulfman, PhD, Karen Knipping, BSc, Dianne J. Delsing, PhD, Edward F. Knol, PhD, Bee Wah Lee, MD  Journal of Allergy and Clinical Immunology  Volume 130, Issue 6, Pages (December 2012) DOI: /j.jaci Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Dose-dependent effect of scGOS on basophil activation. Expression of CD203c on IgEhigh cells was analyzed by using flow cytometry. A, Anti-IgE (1 μg/mL), lcFOS (10 μg/mL), CMF1 without prebiotics (CMF1 w/o prebiotics), indicated doses of CMFs, and scGOSs. B, scGOS fractions DP2, DP3, DP4, DP5, and DP6. C, Patients’ blood was washed with Dulbecco PBS to remove the plasma. The cells were then incubated with scGOS fractions DP2, DP3, DP4, DP5, and DP6 and anti-IgE for 15 minutes at 37°C. MFI, Mean fluorescence intensity. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Dose-dependent effect of scGOS fractions on activation of in vitro–sensitized control basophils. Basophil activation was analyzed by using flow cytometry and is expressed as the percentage of CD63+ cells within the CD203c/CD123+ cells. A, Anti-IgE, standard scGOS, low-protein scGOS, and the scGOS fractions DP2, DP3, DP4, DP5, and DP6 at indicated doses were tested with sera from patients 1, 2, and 3 and a healthy control subject (C). B, Inhibition of scGOS-induced basophil degranulation by the phosphatidylinositol 3-kinase inhibitor wortmannin. Control PBMCs were incubated with sera from patients 1 and 2. After 5 minutes of preincubation with 300 nmol/L wortmannin at 37°C, basophils were stimulated for 30 minutes at 37°C with anti-IgE, N-Formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), low-protein scGOS, and the scGOS fraction DP6 at indicated doses. GOS, Galacto-oligosaccharide. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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