1. SBT Unique Selling Points

2. SBTexcellerator® Single PCR reaction per HLA locus
For HLA Class I coverage of the complete gene
For HLA class II coverage of exon 2 (and exon3)
Primers provided for amplification and sequencing
Core regions
Extended regions to resolve allele ambiguities
GSSP’s to resolve genotype ambiguities 2

3. Amplification strategy
HLA-A (3.1 kb) 1 8 7 6 5 4 3 2
UTR
HLA-B (3.4 kb)
HLA-C (3.4 kb)
HLA-DRB1 ( kb)
HLA-DQB1 ( kb)
HLA-DPB1 (5.0 & 5.7 kb)
HLA-DPA1 (4.7 kb)
HLA-DQA1 ( kb)
HLA-DRB3 (3.8 kb)
HLA-DRB4 (0.4 & 1.3 kb)
HLA-DRB5 (4.0 kb)
SBTexcellerator
amplification primer
Amplified exon
Exon not amplified
Class I amplified entirely
Class II large introns, not all exons amplified.

4. Reagent Box Set-up 2 3 4 5 6 7 8
3’UTR 1
5’UTR
CORE
EXTENDED
FULL 4

5. SBTexcellerator® High priority regions (intron positions)
exclude null alleles
GPS tool resolving genotype ambiguities
to speed up the process
Highest number of GSSP’s per locus
Resolving utmost all genotype ambiguities
Development/manufacturer of reagents en software under 1 roof
Highest level and fast support