1. Fosmid Library Construction Report
Source of RNA
1-1. Sample Information: Paracoccus haeundaensis
1-2. Total genomic DNA Concentration:
NanoDrop® ND-1000 UV-Vis Spectrophotometer
1-3. Electrophoresis of g.DNA:
2. Fosmid Library Construction
2-1. Type of Library: Plasmid library
2-2. Name of Vector: CopyControlTM –pCC1FOSTM
2-3. Insert Ratio: > 100 %
2-4. Insert Size distribution: >40Kb
2-5. Titer: 0.7X 103 pfu/10ul ligate
2-6. Host strain: EPI300-T1R
2-7. Insert Check by Restriction Enzyme Digestion:
Sample
Conc. (ng/ul)
A260/A280
gDNA
177.9
1.9
NEB Mid range PFGE marker
1kb Ladder
CopyControl pCC1FOS Vector
(8.2 kb)
Lane
Sample name
Treatment 1
Low range marker 2
mid range marker 3
10kb Ladder
4~19
Fosmid DNA
NotI
Lane
Sample name
Quantity 1
gDNA
1ug 2
1kb Ladder
Figure 1. Insert Size Determination. 16 colonies are randomly picked and insert DNA are digested
with NotI
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2. Fosmid Library Construction Report
2-8. Vector Information:
Figure 2. CopyControlTM –pCC1FOSTM Vector Map
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