1. Laboratory of Chemistry
TASK 2.1
Analysis of the seaweed biomass
produced in WP1
Laboratory of Chemistry
Pedro Alexandre Reis

2. Task 2.1 – Analysis of the seaweed biomass
produced in WP1
LABORATORY OF CHEMISTRY - MARINNOVA :
Atomic Absorption Spectrometer (AAS): Metals
Skalar Segmented Flow Autoanalyzer (SFA): Nutrients
Gas Chromatography – Mass Spectrometer (GC-MS): PAHs
Liquid Chromatography – Mass Spectrometer (LC-MS): Microcystins and other organic compounds
Isotope Ratio - Mass Spectrometer (IR-MS): 15N/14N and 13C/12C
MORE INFORMATIONS:

3. Task 2.1 – Analysis of the seaweed biomass
produced in WP1
Direct partners: AlgaPlus (Red seaweed: Gracilaria sp.)
CEVA (Green seaweed: Ulva sp.)
CPS (Brown seaweed: Alaria esculenta)
Elements: Metals and Nutrients
Metals:
Essential elements:
Copper (Cu)
Iron (Fe)
Zinc (Zn)
No-essential elements:
Arsenic (As)
Cadmium (Cd)
Chromium (Cr)
Lead (Pb)
Mercury (Hg)
Nutrients:
Ammonium (NH3)
Nitrites (NO2)
Nitrates (NO3)
Total-Nitrogen (N-Total)
Phosphates (PO4)
Total-Phosphorus (P-Total)

4. AAS – Flame Atomization AAS – Electrothermal Atomization
Task 2.1 – Analysis of the seaweed biomass
produced in WP1
Chemical analyses: Metals
Analytical methods: Atomic Absorption Spectrometry (AAS)
AAS – Flame Atomization
AAS – Electrothermal Atomization

5. Task 2.1 – Analysis of the seaweed biomass
produced in WP1
Chemical analyses: Nutrients
Analytical methods: Skalar Segmented Flow Autoanalyzer (SFA)
Skalar San Plus System

6. Task 2.1 – Analysis of the seaweed biomass
produced in WP1
Collection Strategy:
Latex gloves and steril plastic bags
(material isent of metals or nutrients)
Three replicates per sample (N=3)
Minimum mass (fresh weight/per replicate): 50 grams
Storage and Preservation Strategy:
Correct identification of samples: name + date + laboratory
Immediatly freezed at least -20 ºC
Time interval between collection and analyses:
No significant losses or degradation within 4 weeks

7. Dry sample + solution of HNO3 + two-steps of 340W during 4 min
Task 2.1 – Analysis of the seaweed biomass
produced in WP1
Pre-treatment of seaweed samples for metal and nutrients analyses:
Liophylized during 2-3 days (until constant weight)
Reis and Almeida (2008): Acid digestion with high-pressure microwave system
Dry sample + solution of HNO3 + two-steps of 340W during 4 min
Analytical methods for metals and nutrients:
Adapted from certified:
EPA methods
Standard methods for the examination of water and wastewaters (20th edition)
Our analytical methods acreditation: ISO/IEC (initial stage)

8. Task 2.1 – Analysis of the seaweed biomass
produced in WP1
Data Quality Assurance:
Evaluation of analyst performance for each method;
Evaluation of instrument performance for each method: precision, limits of detection and quantification and accuracy;
Analyses of blanks and samples with known additions of metals and nutrients (at each batch of 5% of total samples).
Analyses of Certified Reference Materials (CRM) for metals and nutrients:
DORM-2 (Dogfish Muscle);
TORT-2 (Lobster Hepatopancreas);
SRM 2976 (Mussel Tissue).

9. Pedro Alexandre Reis
CIIMAR - Laboratory of Chemistry
Rua dos Bragas, 289
Porto
Portugal
Phone:

10. Laboratory of Chemistry
TASK 2.1
Analysis of the seaweed biomass
produced in WP1
Laboratory of Chemistry
Pedro Alexandre Reis