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Photosynthesis: Using Light to Make Food
Chapter 7 Photosynthesis: Using Light to Make Food
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Biology and Society: Green Energy
People take advantage of the plant's ability to capture solar energy Wood has historically been the main fuel used to: cook warm homes provide light at night Industrialized societies replaced wood with fossil fuels including coal, gas, and oil. To limit the damaging effects of fossil fuels, researchers are investigating the use of biomass (living material) as efficient and renewable energy sources.
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Biology and Society: Green Energy
Fast-growing trees, such as willows: can be cut every three years; do not need to be replanted; are a renewable energy source, produce fewer sulfur compounds, reduce erosion; provide habitat for wildlife
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Figure 7.0 Figure 7.0 Capturing solar energy 4
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Biology and Society: Biofuels
There are several types of biofuels. Bioethanol is a type of alcohol produced by the fermentation of glucose made from starches in crops such as grains, sugar beets, and sugar cane. Bioethanol may be used directly as a fuel source in specially designed vehicles or as a gasoline additive. Cellulosic ethanol is a type of bioethanol made from cellulose in nonedible plant material such as wood or grass. Biodiesel is made from plant oils or recycled frying oil 5
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THE BASICS OF PHOTOSYNTHESIS
is used by plants, some protists, and some bacteria transforms light energy into chemical energy uses carbon dioxide and water as starting materials The chemical energy produced via photosynthesis is stored in the bonds of sugar molecules. Student Misconceptions and Concerns 1. Students may understand the overall chemical relationships between photosynthesis and cellular respiration, but many struggle to understand the use of carbon dioxide in the Calvin cycle. Photosynthesis is much more than gas exchange. 2. Students may not connect the growth in plant mass to the fixation of carbon during the Calvin cycle. It can be difficult for many students to appreciate that molecules in air can contribute significantly to the mass of plants. Teaching Tips 1. When introducing the diverse ways that plants impact our lives, consider challenging your students to come up with a list of products made from plants that they encounter regularly. Perhaps you might only list those encountered in a single day of college life. The list can be surprising and help to build up your “catalog of examples.” 2. Within the living world, there are many examples of adaptations to increase surface area. Some examples are the many folds of the inner mitochondrial membrane, the highly branched surfaces of fish gills and human lungs, and the highly branched system of capillaries in the tissues of our bodies. Consider relating this broad principle seen elsewhere to the extensive folding of the thylakoid membranes. 3. In our world, energy is frequently converted to a usable form in one place and used in another. For example, electricity is generated by power plants, transferred to our homes, and used to run computers, create light, and help us prepare foods. Consider relating this common energy transfer to the two-stage process of photosynthesis. 4. You might wish to discuss the evolution of chloroplasts from photosynthetic prokaryotes if you will not address this subject elsewhere in your course. 5. Students who have not read all of chapter 7 may not realize that glucose is not the direct product of photosynthesis. Although glucose is shown as a product of photosynthesis, a three-carbon sugar is directly produced (G3P). A plant can use G3P to make many types of organic molecules, including glucose. (The authors address the production of G3P under the section “The Calvin Cycle” later in this chapter.) 6. Figure 7.3 is an important visual organizer that notes the key structures and functions of the two stages of photosynthesis. This figure reminds students where water and sunlight are used in the thylakoid membranes to generate oxygen, ATP, and NADPH. The second step, in the stroma, reveals the use of carbon dioxide, ATP, and NADPH to generate carbohydrates.
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Photosynthetic Protists Photosynthetic Bacteria
Photosynthetic autotrophs: Producer of most ecosystem Organisms that use photosynthesis are: photosynthetic autotrophs the producers for most ecosystems Plants (mostly on land) Photosynthetic Protists (aquatic) PHOTOSYNTHETIC AUTOTROPHS Photosynthetic Bacteria Micrograph of cyanobacteria Kelp, a large alga Forest plants LM Figure 7.1 A diversity of photosynthetic autotrophs
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Chloroplasts: Sites of Photosynthesis
Chloroplasts are the site of photosynthesis; chemical factories powered by the sun; convert solar energy into chemical energy; found mostly in the interior Mesophyll cells of leaves are specialized for photosynthesis, contain many chloroplasts. Stomata are tiny pores where carbon dioxide enters a leaf and oxygen exits. Photosynthetic cells Vein CO2 O2 Stomata Leaf cross section Figure 7.2-1 Figure 7.2 Journey into a leaf (step 1) 8
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Journey into the chloroplast
Structure and Function Inside double-membrane bound of chloroplasts are folded membranous sacs called thylakoids. Thylakoids are concentrated in stacks called grana & are interconnected Grana are suspended in thick viscous fluid, called stroma. Green color of chloroplasts comes from chlorophyll, a light-absorbing pigment that plays a central role in converting solar energy to chemical energy. Journey into the chloroplast Interior cell LM Stroma Granum Thylakoid space Chloroplast Outer membrane TEM Inner membrane Figure 7.2 Journey into a leaf (Step 1)
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Journey into a leaf (step 3)
Photosynthetic cells Chloroplast Inner and outer membranes Vein Thylakoid space Stroma Granum CO2 O2 Stomata LM Leaf cross section Interior cell Colorized TEM Figure 7.2-3 Figure 7.2 Journey into a leaf (step 3) 10
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The Overall Equation for Photosynthesis
Photosynthesis is the process by which autotrophic organisms convert light into chemical energy In the overall equation for photosynthesis, notice that: The reactants of photosynthesis are the waste products of cellular respiration. Carbon dioxide 6 O2 6 CO2 6 H2O C6H12O6 Water Glucose Photo- synthesis Oxygen gas Light energy This simple chemical equation highlights the relationship between photosynthesis and cellular respiration. The reactants of photosynthesis are the waste products of cellular respiration – PS takes the exhaust of CR and rearrange its atoms to produce food and oxygen. During respiration, a fall of e- from food molecules to O2 to form water release the energy that mitochondria can use it to make ATP. The opposite occurs in PS. E- are boosted uphill and added to CO2 to produce sugar. This process requires lot of energy which is supplied by the light energy and e- from hydrogen atom of water molecule
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The Overall Equation for Photosynthesis
In photosynthesis: sunlight provides the energy electrons are boosted “uphill” and added to CO2 sugar is produced water is split into hydrogen and Oxygen Hydrogen which is transferred along with electrons and added to CO2 to produce sugar. Oxygen escapes through stomata into the atmosphere
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Predicting Photosynthetic Process
If photosynthesis is the reverse of cellular respiration, then what processes are needed? Remember that cellular respiration consisted of glycolysis, the citric acid cycle, and the electron transport chain If reversed, there should be a process handling electrons, a cycle that builds organic molecules from CO2, and a reaction that assembles the organic molecules into sugars 1- ETC 2- Cycle 3- Make glucose
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A Photosynthesis Road Map
The initial incorporation of carbon from the atmosphere into organic compounds is called carbon fixation. This lowers the amount of carbon in the air. Deforestation reduces the ability of the biosphere to absorb carbon by reducing the amount of photosynthetic plant life. Photosynthesis occurs in two stages: The light reactions convert solar energy to chemical energy The Calvin cycle uses the products of the light reactions to make sugar from carbon dioxide (energized electrons are added to the CO2 to make sugar) 14
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THE LIGHT REACTIONS: CONVERTING SOLAR ENERGY TO CHEMICAL ENERGY
H2O Chloroplast The light reactions convert solar energy to chemical energy Light Light reactions ATP – – NADPH O2 O2 Figure 7.3-1 Figure 7.3 A road map for photosynthesis (step 1) In LR chlorophyll in thylakoid membrane absorbs solar energy, convert them to chemical energy of ATP and NADPH (e- carrier) Water is split, provides of source of e- and releases O2 gas In DR Uses the products of LR to power the production of sugar from CO2 Enzymes in stroma are used NADPH provides the high energy e- for the reduction of CO2 to glucose 15
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16 H2O CO2 Chloroplast Light NADP+ ADP P Calvin cycle Light
reactions ATP – – NADPH O2 Sugar Figure 7.3-2 Figure 7.3 A road map for photosynthesis (step 2) In LR chlorophyll in thylakoid membrane absorbs solar energy, convert them to chemical energy of ATP and NADPH (e- carrier) Water is split, provides of source of e- and releases O2 gas In DR Uses the products of LR to power the production of sugar from CO2 Enzymes in stroma are used NADPH provides the high energy e- for the reduction of CO2 to glucose 16
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The Nature of Sunlight Sunlight is a type of energy called radiation, or electromagnetic energy. The full range of radiation is called the electromagnetic spectrum. Visible light Wavelength (nm) Radio waves 500 600 750 700 Wavelength = 580 nm Micro- Gamma rays Infrared UV X-rays 10–5 nm 10–3 nm 103 nm 1 nm 106 nm 1 m 103 m Increasing wavelength The distance between the crests of two adjacent waves is called a wavelength. The full range of radiation (from gamma rays [shortest] to radio signals [longest]) is called the electromagnetic spectrum. Electromagnetic energy travels as rhythmic waves, oscillates between electric and magnetic field. The distance between the crests of 2 adjacent waves is called a wavelength. The full range of radiation of very short wavelength of gamma rays to very long wavelength of radiowaves is called the electromagnetic spectrum
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Animation: Light and Pigments
Why are leaves green? Light Reflected light Chloroplast Absorbed light Transmitted light (detected by your eye) Animation: Light and Pigments Figure 7.5 Figure 7.5 Why are leaves green? 18
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The Process of Science: What Colors of Light Drive Photosynthesis?
Observation: In 1883, German biologist Theodor Engelmann saw that certain bacteria living in water tend to cluster in areas with higher oxygen concentrations. Question: Could this information determine which wavelengths of light work best for photosynthesis? Hypothesis: Oxygen-seeking bacteria will congregate near regions of algae performing the most photosynthesis Experiment: Engelmann Laid a string of freshwater algal cells in a drop of water on a microscope slide added oxygen-sensitive bacteria to the drop used a prism to create a spectrum of light shining on the slide
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The Process of Science: What Colors of Light Drive Photosynthesis?
Results: Bacteria: Mostly congregated around algae exposed to red-orange and blue-violet light Rarely moved to areas of green light Conclusion: Chloroplasts absorb specific electromagnetic wavelengths (light mainly in the blue-violet and red-orange part of the spectrum) and convert them to chemical energy. Figure 7.6 Why are leaves green?
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Investigating how light wavelength affects photosynthesis
Prism Microscope slide Bacteria Bacteria Number of bacteria Algal cells 400 500 600 700 Wavelength of light (nm) Figure 7.6 Figure 7.6 Investigating how light wavelength affects photosynthesis 21
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How Sunlight and Chloroplast Pigments Work in Photosynthesis
Chloroplasts contain several pigments to pick up different wavelengths. The most important is chlorophyll. Chlorophyll a: absorbs mainly blue-violet and red light participates directly in the light reactions Chlorophyll b: absorbs mostly blue and orange light participates indirectly in the light reactions
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Chloroplast Pigments Carotenoids:
absorb mainly blue-green light participate indirectly in the light reactions absorb and dissipate excessive light energy that might damage chlorophyll The spectacular colors of fall foliage are due partly to the yellow-orange light reflected from carotenoids. Figure 7.7 Photosynthetic pigments Carotenoids may help protect our eyes from the bright light. The falling autumn temperature causes a decrease in the chlorophyll level
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How Photosystems Harvest Light Energy
Light behaves as photons, discrete packets of energy Photons like electrons have no weight, but travelled very fast, and have a lot of energy Photon collide violently with the pigment molecules inside the chloroplast. Chlorophyll molecules absorb photons electrons in the covalent bonds of the chlorophyll (pigment) gain energy. this energy splits H2O into ½ O2 + 2H+ + 2e- as the electrons fall back to their ground state, energy is released as heat or light. Electrons in the pigment gain energy and become excited – has raised from ground state to excited state – looses its excess energy and falls back to ground state by releasing the heat energy. Some pigments emits light as well as heat energy
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Excited electrons in pigments
A pigment molecule can absorb a photon, causing the pigment's electrons to gain energy Most pigments only release heat energy as their light-executed electrons fall back to ground state Some pigments emit light along with heat after absorbing photons Excited state The electron falls to its ground state. Absorption of a photon excites an electron. e– Heat Light Light (fluorescence) Photon Ground state Chlorophyll molecule (a) Absorption of a photon Figure 7.8 Figure 7.8 Excited electrons in pigments 25
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Breaking a glass vial starts a chemical reactions that excites electrons of a fluorescent dye
The fluorescent light in a glow stick is due to chemical reaction that excites electrons of a fluorescent dye. The excited e- falls back to ground state after releasing the energy in the form of fluorescent light (b) Fluorescence of a glow stick Figure 7.8 Excited electrons in pigments The fluorescent light in a glow stick is due to chemical reaction that excites electrons of a fluorescent dye. The excited e- falls back to ground state after releasing the energy in the form of fluorescent light
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How Photosystems Harvest Light Energy
In the thylakoid membrane, chlorophyll molecules are organized with other molecules into photosystems. A photosystem is a cluster of a few hundred pigment molecules (chlorophyll a, chlorophyll b and some carotenoids ) that function as a light-gathering antenna. The reaction center of the photosystem consists of chlorophyll a molecules that sit next to another molecule called a primary electron acceptor, which traps the light-excited electron from chlorophyll a. Another team of molecules built into the thylakoid membrane then uses that trapped energy to make ATP and NADPH. 27
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A photosystem A photosystem is a group of chlorophyll and other molecules that function as a light-gathering antenna. Chloroplast Cluster of pigment Molecules embedded in membrane Photon Primary electron acceptor Granum (stack of thylakoids) Reaction center Electron transfer e– Reaction- center chlorophyll a Antenna pigment molecules Transfer of energy Thylakoid membrane Photosystem Figure 7.9 Figure 7.9 A photosystem: light-gathering molecules that focus light energy onto a reaction center 28
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THE LIGHT REACTIONS: CONVERTING SOLAR ENERGY TO CHEMICAL ENERGY
Step 1 Chlorophyll in thylakoid membrane absorbs solar energy, This is then converted into chemical energy of ATP (the molecule that drive most of cellular work) and NADPH (electron carrier) Light drives electrons from water to NADP+ (the oxidized form of the carrier) to form NADPH (the reduced form of the carrier) Step 2 Water is split, providing an electrons source and releasing O2 gas Figure 7.6 Why are leaves green?
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How the Light Reactions Generate ATP and NADPH
The light reactions occurred in the thylakoid membrane. Two types of photosystems cooperate in the light reactions: The water-splitting photosystem The NADPH-producing photosystem An electron transport chain: connects the two photosystems releases energy that the chloroplast uses to make ATP
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Light reactions (Step 1)
Primary electron acceptor Water-splitting photosystem Light H2O 2 H Reaction- center chlorophyll 2e – O2 + Photons excite electrons (e-) in the chlorophyll of Water-splitting photosystem (WSP). The electrons are then trapped by the Primary electron acceptor. The WSP replaces its light –exited electrons by extracting electrons from water (release of O2) Figure 7.10 Light reactions (Step 1)
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Light reactions (Step 2)
Energy to make Those energized e- from WSP pass down the ETC to the NADPH-producing photosystem. The chloroplasts uses the energy released by this electron “fall” to make ATP ATP Primary electron acceptor 2e- Electron transport chain Light Reaction- center chlorophyll H2O 2e – Water-splitting photosystem 2 H + + O2 Figure 7.10 Light reactions (Step 2) 2. Energised e- from PS II pass down the ETC to the NADPH-producing PS. The chloroplast uses the energy released by this e- fall to make ATP
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Light reactions (Step 3)
Primary electron acceptor NADP 2e – Energy to make ATP Primary electron acceptor 2e – NADPH 2e – Light e- boosted to Electron transport chain Light Series of Redox Reaction Reaction- center chlorophyll Reaction- center chlorophyll NADPH-producing photosystem H2O 2e – Water-splitting photosystem The NADPH-producing photosystem transfers its light-exited e- to NADP+, reducing it to NADPH. 2 H + + O2 Two places where energy is made Figure 7.10 Light reactions (Step 3) 3. The NADPH-producing PS transfers its light-excited e- to NADP+ to, reducing it to NADPH
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How the thylakoid membrane converts light energy to the chemical energy of NADPH and ATP
– O2 ATP NADP Stroma Inside thylakoid Photosystem Electron transport chain NADPH ADP P H+ synthase To Calvin cycle H Electron flow This figure shows the location of light reaction in the thylakoid membrane This figure shows the location of light reaction in the thylakoid membrane
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35 Stroma Thylakoid membrane Inside thylakoid Thylakoid membrane H
Electron transport chain Thylakoid membrane Photosystem Inside thylakoid 2e – H2O H O2 Thylakoid membrane Figure 7.11a Figure 7.11 How the thylakoid membrane converts light energy to the chemical energy of NADPH and ATP (part 1) 35
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36 To Calvin cycle Light ATP synthase Electron flow H NADPH ATP ADP
Figure 7.11b To Calvin cycle Light – – H NADPH ATP ADP P NADP H Electron transport chain Photosystem ATP synthase Electron flow H H H H H Figure 7.11 How the thylakoid membrane converts light energy to the chemical energy of NADPH and ATP (part 2) 36
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A hard-hat analogy for the light reactions
– ATP e – e – – – NADPH e – e – e – Photon e – Photon Water-splitting photosystem NADPH-producing photosystem Figure 7.12 Figure 7.12 A hard-hat analogy for the light reactions 37
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In summary, light reactions take place in the thylakoid membranes
Products are NADPH ATP Oxygen.
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THE CALVIN CYCLE: MAKING SUGAR FROM CARBON DIOXIDE
Calvin Cycle utilizes the products of the light reaction to create sugar from carbon dioxide Inputs are three CO2 , energy from ATP and high energy electrons from NADPH. Carbon dioxide (CO2) enters the cycle An enzyme adds CO2 to a five-carbon sugar (RuBP) Resulting molecule breaks into two, 3-carbon molecules Energy from the produced ATP and NADPH is utilized Enzymes convert each three-carbon molecule to the three-carbon sugar G3P For every 3 molecules of CO2 that enter the cycle, the net output is one G3P sugar as raw material for making glucose and other organic compounds and the starting material to continue the cycle Other G3P sugars continue in the cycle Utilizing energy from ATP, enzymes rearrange the leftover G3P sugars to regenerate RUBP (which will be used in the next cycle) It is called cycle because the starting material is regenerated with each turn of the cycle. Inputs are CO2 , energy from ATP and high energy e- from NADPH. Output G3P as raw material for making glucose and other organic compounds and the starting material to continue the cycle
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Calvin cycle (Step 1) Calvin cycle
Three-carbon molecule RuBP sugar CO2 (from air) An enzyme adds each Co2 to a 5-C sugar called RuBP and the resulting molecule breaks into two 3-C molecules An enzyme adds each Co2 to a 5-C sugar called RuBP and the resulting molecule breaks into two 3-C molecules
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Calvin cycle (Step 2) Calvin cycle
CO2 (from air) P RuBP sugar Three-carbon molecule ATP P P ADP P Calvin cycle NADPH NADP G3P sugar P Using energy from ATP and NADPH produced by the LR, enzymes convert each 3-C molecule to the 3-C sugar (G3P) The Calvin cycle (Step 2) Using energy from ATP and NADPH produced by the LR, enzymes convert each 3-C molecule to the 3-C sugar (G3P)
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Calvin cycle (Step 3) CO2 (from air) P RuBP sugar Three-carbon molecule ATP P P ADP P Calvin cycle For every 3 molecules of CO2 that enters the cycle, the net output is one G2P sugar. The other G3P sugars continue in the cycle NADPH NADP G3P sugar G3P sugar P P G3P sugar Glucose (and other organic compounds) P Figure The Calvin cycle (Step 3) For every 3 molecules of CO2 that enters the cycle, the net output is one G2P sugar. The other G3P sugars continue in the cycle
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CO2 (from air) Calvin cycle (Step 4) P RuBP sugar Three-carbon molecule ATP P P ADP P ADP P Calvin cycle NADPH ATP NADP G3P sugar G3P sugar P P Using energy from ATP, enzymes rearrange the remaining G3P sugars to regenerate RuBP G3P sugar Glucose (and other organic compounds) P Figure The Calvin cycle (Step 4) Using energy from ATP, enzymes rearrange the remaining G3P sugars to regenerate RuBP
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Summary: Calvin cycle NADPH Calvin cycle ADP P NADP ATP G3P CO2 Glucose and other compounds It takes two turns of Calvin cycle to produce one molecule of glucose Figure 7.UN7 Summary: Calvin cycle
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Evolution Connection: Solar-Driven Evolution
Other alternative mode of Photosynthesis C3 plants: the first organic compound produced in the Calvin Cycle is a three-carbon molecule (soybeans, oats, rice) use CO2 directly from the air ; very common and widely distributed; close their stomata on hot/dry days (prevents dehydration; prevents CO2 from entering the leaves; CO2 levels get very low in the leaves; sugar production ceases); in hot weather, C3 production decreases significantly C4 plants:(Corn, sugarcane) close their stomata to save water during hot and dry weather can still carry out photosynthesis CAM plants: (pineapple, cacti, Aloe) are adapted to very dry climates open their stomata only at night to conserve water So far we learned that Plants convert solar energy to chemical energy via PS Plants use certain wavelength of light to drive these reactions How do plants synthesize food under hot, dry weather? Alternative mode of incorporating carbon from CO2 have evolved in some plants that allows them to save water without shutting down PS
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C4 plants Incorporate carbon from CO2 into a 4-C compounds an enzyme continue to incorporate C even when a leaf's CO2 concentration is low - two types of cells are involved cell type 1(mesophyll cell) and cell type 2 ( bundle sheath cell) Sugar C4 Pathway (example: sugarcane) C4 plant CAM plant Calvin cycle Day Cell type 1 Four-carbon compound Night type 2 CAM Pathway (example: pineapple) ALTERNATIVE PHOTOSYNTHETIC PATHWAYS CO2 CAM plants Incorporate carbon from CO2 into a 4-C compounds at night Release the 4-C compounds to Calvin cycle during day Figure 7.14 C4 and CAM photosynthesis C4 plants incorporate C from CO2 into a 4-C compounds even when the leaf CO2 level is low. 2 types of cells – mesophyll cell and bundle sheath cell: mesophyll incorporates C in 4-C compound and send it to BSC
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Review: Photosynthesis equation
Light energy 6 CO2 6 H2O C6H12O6 6 O2 Photo- synthesis Carbon dioxide Water Glucose Oxygen gas Figure 7.UN1 Photosynthesis equation
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Review: light reaction
Figure 7.UN2 Orientation diagram: light reactions
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Review: light reaction
reactions CO2 O2 H2O (C6H12O6) NADPH Sugar ATP ADP P NADP Calvin cycle Figure 7.UN2 Orientation diagram: light reactions
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Summary: light reaction
NADPH-producing photosystem Water-splitting photosystem Figure 7.UN6 Summary: light reactions
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Summary: light reaction
NADP+ e – 2e – ADP ATP acceptor e – 2e – acceptor 2e NADPH – Photon Electron transport chain Photon Chlorophyll H2O Chlorophyll NADPH-producing photosystem 2e – Water-splitting photosystem 2 H 2 1 + O2 + Figure 7.UN6 Summary: light reactions
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Review: Calvin cycle Figure 7.UN3 Orientation diagram: Calvin cycle
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Summary of Key Concepts: A Photosynthesis Road Map
Photosynthesis is the older process evolutionary compared to Cellular respiration (WHY?) Chloroplast CO2 Light H2O Stroma Stack of thylakoids NADP RuBP ADP 3-PGA P Calvin cycle Light reactions ATP – – G3P sugar NADPH Sugar used for cellular respiration cellulose starch other organic compounds O2 Sugar (C6H12O6) Figure 7-UN05 Summary of Key Concepts: A Photosynthesis Road Map 53
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Review: Calvin cycle CO2 H2O NADP ADP Calvin Light cycle reactions
(C6H12O6) NADPH Sugar ATP ADP P NADP Calvin cycle Figure 7.UN3 Orientation diagram: Calvin cycle
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