1. SD Malaria P.f/P.v Antigen Test STANDARD DIAGNOSTICS, INC.
ISO 9001 Certified Company
SD Malaria P.f/P.v Antigen Test
STANDARD DIAGNOSTICS, INC.

2. ISO 9001 Certified Company
What is Malaria ?
Anopheles Mosquito
1. Malaria is caused by a protozoan (Plasmodium sp.) which invades human red blood cells
2. The parasite is usually transmitted to humans by the bite of the female Anopheles mosquito

3. Where is Malaria ? 1. 90 countries worldwide
ISO 9001 Certified Company
Where is Malaria ?
1. 90 countries worldwide
2. Almost 85% of the world's malaria occurs in sub-Saharan Africa.
3. Malaria is also prevalent in Southeast Asia, India, South and Central America

4. How suffered? - Fatal, parasitic disease characterized
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How suffered?
- Fatal, parasitic disease characterized
by fever, chills, and anemia
- Over 500 million clinical cases and
2.7 million malaria-caused deaths
per year
[A young mother who has bought her sick child – Uganda]

5. SD Malaria P.f/P.v Ag Test
ISO 9001 Certified Company
SD Malaria P.f/P.v Ag Test
Rapid Test
Detection of pLDH
ELISA Kit
pLDH
RBC
Antibody to pLDH

6. SD Malaria P.f/P.v Ag Rapid Test
ISO 9001 Certified Company
SD Malaria P.f/P.v Ag Rapid Test
Rapid and Easy
Detection of all forms
of human malaria
Differentiation between P. f /P. v
Direct Sandwich Method: Ab-Ag-Ab
Ability to monitor of drug-therapy

7. Differentiation between P. f /P. v
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Differentiation between P. f /P. v
pLDH is isoenzyme of all Plasmodium
species.
Use of P.f/P.v Pan Antibody and P.f specific
antibody to pLDH
 Able to differentiate between P.f/P.v
Disable to differentiate 
Why? The HRP-2 only exists in P.f
pLDH
HRP-2

8. Easy test procedure of Rapid test
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Easy test procedure of Rapid test
1. 1 drop of Assay Buffer into the Conjugate Well
2. 4 drops of Assay Buffer into the Washing Well
3. Add 20µl of blood the Conjugate Well
4. Place the Dipstick vertically into the Conjugate Wells
5. Transfer the Dipstick from the conjugate Wells to the Washing Wells
6. Read the results

9. Interpretation of the result
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Interpretation of the result
P. falciparum +
P. vivax +
or P. malariae +
or P. ovale +
Negative
Invalid

10. SD Malaria P.f/P.v pLDH Ag ELISA Kit
ISO 9001 Certified Company
SD Malaria P.f/P.v pLDH Ag ELISA Kit
The First ELISA kit in the World
The Only Malaria Ag ELISA in the World
Direct Sandwich Method: Ab-Ag-Ab
Ability to monitor of drug-therapy
Excellent detection limit
10 ~50 parasites/ul
Reliable alternative to Microscopy

11. Easy test procedure of ELISA Kit
ISO 9001 Certified Company
Easy test procedure of ELISA Kit
⇒ The SD Malaria P.f/P.v Ag ELISA contains two microplates.
- One is uncoated for lysis of whole blood,
- The other is pre-coated with mouse pLDH antibody on well.
⇒ After lysing whole blood in uncoated well,
The test is performed as like a general ELISA kit

12. Comparison of test method regarding to No. of parasite
ISO 9001 Certified Company
Comparison of test method
regarding to No. of parasite

13. Comparison of test method regarding to No. of parasite
ISO 9001 Certified Company
Comparison of test method
regarding to No. of parasite

14. Comparison of test method regarding to No. of parasite
ISO 9001 Certified Company
Comparison of test method
regarding to No. of parasite

15. Comparison of test method
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Comparison of test method
No. of Positive
No. of Negative
Sensitivity(%)
Specificity(%)
Microscopic Exam.
200
SD ELISA kit
194
197
97.0%
98.5%
SD Rapid test
181
196
90.5%
98.0%
A Rapid A
(Detection for pLDH)
179
198
89.5%
99.0%
A Rapid B
(Detection for HRP-2)
172
86.0%