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Somatic Mutation of the 5′ Noncoding Region of the BCL-6 Gene Is Associated with Intraclonal Diversity and Clonal Selection in Histological Transformation.

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Presentation on theme: "Somatic Mutation of the 5′ Noncoding Region of the BCL-6 Gene Is Associated with Intraclonal Diversity and Clonal Selection in Histological Transformation."— Presentation transcript:

1 Somatic Mutation of the 5′ Noncoding Region of the BCL-6 Gene Is Associated with Intraclonal Diversity and Clonal Selection in Histological Transformation of Follicular Lymphoma  Zoltán Szereday, Balázs Csernus, Monika Nagy, Terézia László, Roger A. Warnke, András Matolcsy  The American Journal of Pathology  Volume 156, Issue 3, Pages (March 2000) DOI: /S (10) Copyright © 2000 American Society for Investigative Pathology Terms and Conditions

2 Figure 1 PCR-SSCP analysis of the 5′ noncoding regions of BCL-6 gene in paired serial biopsy specimens of FL (A) and transformed DLCL (B). Representative results obtained for PCR-amplified fragments from the E1.11 regions of BCL-6 gene in Cases 3–5 are shown. A positive control (P), represented by a tumor sample known to harbor BCL-6 mutations, as well as sample that was not denatured before electrophoresis (N) are shown. Arrows identify abnormally migrating bands. The American Journal of Pathology  , DOI: ( /S (10) ) Copyright © 2000 American Society for Investigative Pathology Terms and Conditions

3 Figure 2 Schematic representation of the BCL-6 5′ noncoding region diversification and sequence intraclonal heterogeneity in FL (A) and transformed DLCL (B) samples. Each sequence is represented as a horizontal line. The first nucleotide of the BCL-6 cDNA is designated as position +1. The arrows represent the positions of the primers. Within each sequence, nucleotide differences from the BCL-6 cDNA sequences are indicated by dots. The type and exact positions of the mutations are specified in Table 2. The American Journal of Pathology  , DOI: ( /S (10) ) Copyright © 2000 American Society for Investigative Pathology Terms and Conditions

4 Figure 3 Probable clonal relationship of FL and transformed DLCL clones in Case 3. The genealogical tree is constructed from the pattern of shared and unique mutations of E1.13 PCR fragment of the BCL-6 5′ noncoding sequences (Table 2 and Figure 2), assuming that shared mutations represent single events. The FL clones (A) are drawn as open circles and DLCL clones (B) are drawn as shaded circles. The number of mutations that separate each branch is given. The American Journal of Pathology  , DOI: ( /S (10) ) Copyright © 2000 American Society for Investigative Pathology Terms and Conditions

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