1. A new serum proteolytic enzyme in aneurysm pathogenesis
Spencer L. Brown, M.D., Birgitta Backstrom, Ronald W. Busuttil, M.D., Ph.D. 
Journal of Vascular Surgery 
Volume 2, Issue 3, Pages (May 1985)
DOI: / (85)
Copyright © 1985 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

2. Fig. 1
Comparative elastase activity of human PMNs (106/ml), mononuclear cells (106/ml), and serum from patient with aneurysm, with use of S-2484, specific for leukocyte elastase. (See Methods section for details.)
Journal of Vascular Surgery 1985 2, DOI: ( / (85) )
Copyright © 1985 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

3. Fig. 2
Fractionation of serum on Sepharose CL-6B column. Five milliliters of serum were added to column and contents were eluted by use of 0.1 M Tris with 0.5 M NaCl (pH 8.0). Peak 1: serum substance that retains SANA proteolytic activity; peak 2: serum immunoglobulin; peak 3: albumin.
Journal of Vascular Surgery 1985 2, DOI: ( / (85) )
Copyright © 1985 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

4. Fig. 3
Retained substance from ultrafiltration membrane XM-100A eluted on Sepharose CL-6B column. Filter excludes substances of MW >100,000 daltons. Peak 1: purified active compound that retains SANA proteolytic activity; peak 2: residual albumin.
Journal of Vascular Surgery 1985 2, DOI: ( / (85) )
Copyright © 1985 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

5. Fig. 4
Treatment of active serum proteolytic substance isolated from XM-100A filter with Triton X-100 and elution on Sepharose CL-6B column. Detergent Triton X-100 splits active enzyme into at least four components of different MWs: 310,000, 62,000, 40,000, and 10,000 daltons.
Journal of Vascular Surgery 1985 2, DOI: ( / (85) )
Copyright © 1985 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

6. Fig. 5
Optimum pH curve of serum proteolytic compound. Enzyme is most active between pH 7.0 to 8.0.
Journal of Vascular Surgery 1985 2, DOI: ( / (85) )
Copyright © 1985 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions