1. PHYTOCHEMICAL ANALYSIS OF THE POLYPHENOLS OF ONONIS ARVENSIS L.
ANALIZA FITOCHIMICĂ A POLIFENOLILOR DIN ONONIS ARVENSIS L.
Dénes Tünde1, Boros Borbála2, Felinger Attila2,3, Varga Erzsébet4, Papp Nóra1
1Department of Pharmacognosy, University of Pécs, Rókus u. 2., H-7624 Pécs, Hungary
2Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Ifjúság útja 6, H-7624 Pécs, Hungary
3MTA–PTE Molecular Interactions in Separation Science Research Group, Ifjúság útja 6, H-7624 Pécs, Hungary
4Department of Pharmacognosy and Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy, Targu Mures, Gh. Marinescu 38, Romania 
OBJECTIVES
MATERIAL AND METHODS
Traditional ethnomedicinal knowledge about plant use can be attributed to the archaic folklore system in Romania. Several surveys are being performed nowadays, focusing on the study of new plant taxa. Based on comparison with previous reports (Spilková et al. 1982, 2001), field restharrow (Ononis arvensis L., Fabaceae) was selected in our field work in Homoród-valley for further studies.
We have collected ethnobotanical data in 5 villages of Homoród-valley (Crăciunel, Mărtiniș, Merești, Petreni, Sânpaul) in summer of The herb of Ononis arvensis (Ononidis arvensis herba) was mentioned for diarrhoea, liver and stomach disorders as tea.
The aerial parts of the species was collected at hayfield in Petreni in July Polyphenolic compounds were studied by Q-TOF LC/MS with ESI ion source. MS detection was performed on Ascentis Express C18 column. The aglycone content was determined based on the monography of Crataegi folium cum florae, the total flavonoids according to Cynarae folium (Farmacopeea Română 1993).
Polyphenolic compounds
LOD
(ng mL-1)
LOQ
Catechin
16.85
55.61
Epicatechin
19.81
65.38
Ellagic acid
7.03
23.21
Caffeic acid
67.39
222.40
Chlorogenic acid
5.06
16.70
Kaempherol
0.34
1.12
p-Coumaric acid
Abscisic acid
17.59
58.05
Ferulic acid
10.12
33.40
Quercetin
41.81
137.97
Dihydro-Quercetin
0.96
3.16
Phloridzin
4.87
16.06
Rutin
3.68
12.13
Luteolin
7.04
23.22
Eriodictiol
0.68
2.23
Gallic acid
44.15
145.69
Naringenin
0.84
2.77
trans-Resveratrol
17.97
59.31
Piceid
7.85
25.90
Apigenin
1.27
4.18
Table 1.
LOD and LOQ
results for
polyphenolic
standards
Plant collection in field trip
Ononis arvensis L.
RESULTS
Eighteen polyphenolic compounds were identified and quantified in the aerial parts of O. arvensis (Table 1). The dominant polyphenolic compound was eriodictiol. Phenolic acids (gallic acid, ferulic acid, caffeic acid, ellagic acid and their derivatives, chlorogenic acid, p-coumaric acid) were also detected, as well as flavanones (naringenin, eriodictyol, dihydroquercetin), flavones (apigenin, luteolin), flavonols (quercetin, kaempherol, rutin), flavan-3-ols (catechin, epicatechin), a stilbenoid (trans-resveratrol), a dihydrochalcon (phloridzin), and a stilbenoid glucoside (piceid). The aglycone content was %, total flavonoid content g cm-3.
CONCLUSIONS
REFERENCES
These preliminary results will be completed by other analytical methods of the substances of O. arvensis which can give new phytochemical data about the species.
Spilková J, Bednár P, Stroblíková R. (2001) Capillary electrophoretic analysis of hydroxycinnamic acids from Ononis arvensis L. Pharmazie.
Spilková J, Hubík J. (1982) [Pharmacognosy study of Ononis arvensis L. II. Flavonoids and onocerin in the drug]. Cesk Farm.
Farmacopeea Română, Ed. a X-a, Ed. Medicală, București, 1993