1. From: Inhalational Anesthetics Disrupt Postsynaptic Density Protein-95, Drosophila Disc Large Tumor Suppressor, and Zonula Occludens-1 Domain Protein Interactions Critical to Action of Several Excitatory Receptor Channels Related to Anesthesia
Anesthes. 2015;122(4): doi: /ALN
Figure Legend:
Halothane and isoflurane dose-dependently disrupt postsynaptic density protein-95, Drosophila disc large tumor suppressor, and zonula occludens-1 (PDZ) domain–mediated interactions between postsynaptic density protein-95 (PSD-95) and potassium channel Kv1.4 in an yeast two-hybrid system. (A and B) The effect of halothane on the growth of yeast cells harboring pGADT7-PSD-95 PDZ2 and pGBKT7-Kv1.4 CT100 (n = 6). (A) Halothane dose-dependently inhibited the yeast growth. (B) Yeast growth is shown as a percent of control, relative to halothane concentration. *P < 0.05 and **P < 0.01 versus 0.00 mM halothane. (C−E) The effect of isoflurane on the growth of yeast cells harboring pGADT7-PSD-95 PDZ2 and pGBKT7-Kv1.4 CT100 (n = 3). (C) Isoflurane dose-dependently inhibited the yeast growth. (D) Yeast growth is shown as a percent of control, relative to isoflurane concentration. *P < 0.05 versus 0.00 mM isoflurane. (E) Binned scatter plots of C. CT100 = C-terminal 100 amino acids.
Date of download: 11/5/2017
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