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Development of immunologic memory against tetanus toxoid and pertactin antigens from the diphtheria-tetanus-pertussis vaccine in atopic versus nonatopic.

Published byRalph Nelson Modified over 6 years ago

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Presentation on theme: "Development of immunologic memory against tetanus toxoid and pertactin antigens from the diphtheria-tetanus-pertussis vaccine in atopic versus nonatopic."— Presentation transcript:

1 Development of immunologic memory against tetanus toxoid and pertactin antigens from the diphtheria-tetanus-pertussis vaccine in atopic versus nonatopic children  Patrick G. Holt, DSc, Anna Rudin, PhD, Claudia Macaubas, PhD, Barbara J. Holt, BSc, Julie Rowe, PhD, Richard Loh, FRACP, Peter D. Sly, FRACP  Journal of Allergy and Clinical Immunology  Volume 105, Issue 6, Pages (June 2000) DOI: /mai Copyright © 2000 Mosby, Inc. Terms and Conditions

2 Fig. 1 Cytokine responses to mitogen and allergen in PBMCs from 6-year-old children. PBMCs were stimulated with either HDM (top) or PHA (bottom) as detailed in Methods, and culture supernatants were harvested at 48 hours for ELISAs. Data shown are group means ± SE. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

3 Fig. 2 Vaccine antigen-specific IgG antibody in serum. Antibody levels were determined as detailed in Methods. Data shown are group means ± SE. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

4 Fig. 3 Cytokine responses to tetanus toxoid antigen in PBMCs from 6-year-old children. PBMCs were stimulated with tetanus toxoid antigen, and ensuing cytokine responses were determined as cytokine protein (by ELISA) or cytokine-specific mRNA relative to mRNA specific for the housekeeping gene β-actin (by semiquantitative RT-PCR), as detailed in Methods. Data shown are group means ± SE. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

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