1. Downregulation of eNOS in a nutritional model of fatty liver
Oren Tirosh, Erez Ilan, Noga Budick-Harmelin, Giuliano Ramadori, Zecharia Madar 
European e-Journal of Clinical Nutrition and Metabolism 
Volume 4, Issue 2, Pages e101-e104 (April 2009)
DOI: /j.eclnm
Copyright © 2009 European Society for Clinical Nutrition and Metabolism Terms and Conditions

2. Fig. 1
Following treatment with lipid emulsion (LE) or phosphate-buffered saline (PBS) liver histological sections and staining was performed. Microscopic photos were taken at 400× magnification. Hematoxylin and eosin staining: (A) control rats infused with PBS for 6 days, and (B) LE-infused rats for 6 days. Nile red lipid staining: (C) rats infused with PBS or (D) LE for 6 days (magnification ×100). Photos are representative of blinded histological liver sections prepared from the livers of three animals of control and LE-treated groups.
European e-Journal of Clinical Nutrition and Metabolism 2009 4, e101-e104DOI: ( /j.eclnm )
Copyright © 2009 European Society for Clinical Nutrition and Metabolism Terms and Conditions

3. Fig. 2
Lipid peroxidation levels evaluated by 4-hydroxynonenal-protein adduct levels in rats liver following infusion of LE. Control rats were infused with PBS (n=6) or LE (n=8) for 6 days. Animals were sacrificed at the end of the experiment and liver homogenates were analyzed using specific antibodies against 4HNE-protein adduct by western blot. Data were normalized to total protein. *Indicates statistically significant difference (P<0.05) by Student's t-test.
European e-Journal of Clinical Nutrition and Metabolism 2009 4, e101-e104DOI: ( /j.eclnm )
Copyright © 2009 European Society for Clinical Nutrition and Metabolism Terms and Conditions

4. Fig. 3
Evaluation of eNOS expression in livers of animals treated with LE infusion. (A) Liver eNOS protein levels from control rats (infused with PBS) or rats treated with LE for: day 1 (n=3), day 2 (n=3), day 4 (n=4), and day 6 (n=6). n=Number of LE-treated animals. *Indicates significantly different (P<0.05) from control animals infused with phosphate-buffer saline (PBS) for 6 days. Statistical analysis was performed by one-way ANOVA using post hoc Tukey–Kramer method for multiple groups. (B) mRNA levels evaluated by real-time PCR in control rats (infused with PBS) (n=6) or LE-treated rats (n=8) for 6 days. *Indicates statistically significant difference (P<0.05) by Student's t-test.
European e-Journal of Clinical Nutrition and Metabolism 2009 4, e101-e104DOI: ( /j.eclnm )
Copyright © 2009 European Society for Clinical Nutrition and Metabolism Terms and Conditions