1. Bacteriology Specimen Collection and Processing
Lesson 7-2
Bacteriology Specimen Collection and
Processing

2. Overview Importance of collection and transport methods
Types of cultures commonly performed
Throat
Nasal
Urine
Wound
Respiratory
Stool
Blood

3. Specimen Collection Reasons for ordering cultures
See Table 7-10
Specimen collection manual
Specimen collection method
See Table 7-11

4. Collection and Transport Supplies
Support a variety of specimens
Support anaerobic and aerobic organisms
Protect viability during transport
Approved leak-proof containers
See Table 7-12

5. Collection and Transport Supplies

6. Specimen Collection Safety precautions Quality assessment
Standard Precautions
Disinfect setup area
Quality assessment
Follow procedure manual
Label appropriately
See Table 7-13

7. Specimen Collection Throat culture Nasal culture Swab tonsils only
Inoculate plate or use transport media
Nasal culture
MRSA carriers
Bordetella pertussis
Other

8. Specimen Collection

9. Specimen Collection Urine culture Midstream, clean-catch
Transport system

10. Specimen Collection Wound culture Aspirate preferred
Immediately inoculate transport medium
Culture for anaerobes and aerobes

11. Specimen Collection Respiratory culture – sputum
Rinse mouth before collection
Klebsiella pneumoniae
Streptococcus pneumoniae

12. Specimen Collection Stool culture Specimen must not be uncontaminated
Special media to support
Salmonella, Shigella, E. coli
Vibrio
Campylobacter
Clostridium difficile

13. Specimen Collection Blood culture Phlebotomist wears sterile gloves
Cleanse site with alcohol then iodine
Various styles of collection bottles
This project was funded at $3,000,000 (100% of its total cost) from a grant awarded under the Trade Adjustment Assistance Community College and Career Training Grants, as implemented by the U.S. Department of Labor’s Employment and Training Administration. Rogue Community College is an equal opportunity employer/program. Auxiliary aids and services, alternate form and language services are available to individuals with disabilities and limited English proficiency free of cost upon request.
This work is licensed under a Creative Commons Attribution 4.0 International License.