1. Prolactin role in the bovine uterus during adenomyosis
M. Łupicka, B.M. Socha, A.A. Szczepańska, A.J. Korzekwa 
Domestic Animal Endocrinology 
Volume 58, Pages 1-13 (January 2017)
DOI: /j.domaniend
Copyright © 2016 Elsevier Inc. Terms and Conditions

2. Fig. 1
Messenger ribonucleic acid (mRNA) expression of PRL (A), lPRLR (B), and sPRLR (C) in uterine tissues obtained from control cows and from cows with adenomyosis (ADENO), determined by real-time PCR. Data were normalized against GAPDH. Bars represent the mean ± standard error of the mean. *P < 0.05 indicates statistical difference between uterine control and adenomyotic tissues, as determined by Student's t-test. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PRL, prolactin; lPRLR, long form of PRL receptor; sPRLR, short form of PRL receptor.
Domestic Animal Endocrinology  , 1-13DOI: ( /j.domaniend )
Copyright © 2016 Elsevier Inc. Terms and Conditions

3. Fig. 2
Protein expression of PRL (A), lPRLR (B), and sPRLR (C), and lPRLR:sPRLR protein ratio (D) in uterine tissues obtained from control cows and from cows with adenomyosis (ADENO), determined by Western blotting. Data were normalized against GAPDH. Bars represent the mean ± standard error of the mean. *P < 0.05, **P < 0.01 indicate statistical difference between normal uterine and adenomyotic tissues, as determined by Student's t-test. Representative blots for PRL, PRLRs, and GAPDH are shown below the graphs (E). Mammary gland tissue was used as positive control. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MM, molecular weight marker; PRL, prolactin; lPRLR, long form of PRL receptor; sPRLR, short form of PRL receptor.
Domestic Animal Endocrinology  , 1-13DOI: ( /j.domaniend )
Copyright © 2016 Elsevier Inc. Terms and Conditions

4. Fig. 3
Immunodetection of PRL (A–H) and PRLRs (I–P) in uterine tissues of cows without adenomyosis (control) and cows with adenomyosis (ADENO). Arrowheads indicate the most intense immunoreaction; (R) and (T): negative controls without primary antibodies; (Q) and (S): negative controls with nonspecific IgG. gl, uterine glands; le, luminal epithelium; myo, myometrium; PRL, prolactin; PRLR, PRL receptor; s, stroma; v, blood vessels. Scale bars: 50 μm.
Domestic Animal Endocrinology  , 1-13DOI: ( /j.domaniend )
Copyright © 2016 Elsevier Inc. Terms and Conditions

5. Fig. 4
Messenger ribonucleic acid (mRNA) expression of PRL (A–C), lPRLR (D–F), and sPRLR (G–I) in cultured uterine epithelial, stromal, and myometrial cells isolated from cows without adenomyosis (white bars) and from cows with adenomyosis (black bars) and under the influence of estradiol (E2), determined by real-time PCR. Data were normalized against GAPDH. Bars represent the mean ± standard error of the mean. #P < 0.05, ##P < 0.01, and ###P < indicate statistically significant differences between untreated and treated cells within each experimental group (control or adenomyotic); *P < 0.05, **P < 0.01, and ***P < indicate differences between normal uterine and adenomyotic cells within the untreated or E2-treated group. Statistically significant differences were determined using 2-way analysis of variance, followed by Bonferroni's posthoc test. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PRL, prolactin; lPRLR, long form of PRL receptor; sPRLR, short form of PRL receptor.
Domestic Animal Endocrinology  , 1-13DOI: ( /j.domaniend )
Copyright © 2016 Elsevier Inc. Terms and Conditions

6. Fig. 5
Protein expression of PRL (A–C), lPRLR (D–F), sPRLR (G–I), and lPRLR:sPRLR protein ratio (J–L) in cultured uterine cells isolated from cows without adenomyosis (white bars) and from cows with adenomyosis (black bars) and under the influence of estradiol (E2), determined by Western blotting. Data were normalized against GAPDH. Bars represent the mean ± standard error of the mean. #P < 0.05, ###P < 0.001, ####P < indicate statistically significant differences between untreated and treated cells within each experimental group (control or adenomyotic); *P < 0.05, **P < 0.01, ***P < 0.001, ****P < indicate differences between uterine normal and adenomyotic cells within the untreated or E2-treated group. Statistically significant differences were determined using 2-way analysis of variance, followed by Bonferroni's posthoc test. Representative blots for PRL, PRLRs, and GAPDH are shown below the graphs (M). MM: molecular weight marker; Control: tissues obtained from nonadenomyotic cows; ADENO: tissues obtained from cows with adenomyosis. Mammary gland tissue was used as positive control. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PRL, prolactin; lPRLR, long form of PRL receptor; sPRLR, short form of PRL receptor.
Domestic Animal Endocrinology  , 1-13DOI: ( /j.domaniend )
Copyright © 2016 Elsevier Inc. Terms and Conditions

7. Fig. 5
Protein expression of PRL (A–C), lPRLR (D–F), sPRLR (G–I), and lPRLR:sPRLR protein ratio (J–L) in cultured uterine cells isolated from cows without adenomyosis (white bars) and from cows with adenomyosis (black bars) and under the influence of estradiol (E2), determined by Western blotting. Data were normalized against GAPDH. Bars represent the mean ± standard error of the mean. #P < 0.05, ###P < 0.001, ####P < indicate statistically significant differences between untreated and treated cells within each experimental group (control or adenomyotic); *P < 0.05, **P < 0.01, ***P < 0.001, ****P < indicate differences between uterine normal and adenomyotic cells within the untreated or E2-treated group. Statistically significant differences were determined using 2-way analysis of variance, followed by Bonferroni's posthoc test. Representative blots for PRL, PRLRs, and GAPDH are shown below the graphs (M). MM: molecular weight marker; Control: tissues obtained from nonadenomyotic cows; ADENO: tissues obtained from cows with adenomyosis. Mammary gland tissue was used as positive control. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PRL, prolactin; lPRLR, long form of PRL receptor; sPRLR, short form of PRL receptor.
Domestic Animal Endocrinology  , 1-13DOI: ( /j.domaniend )
Copyright © 2016 Elsevier Inc. Terms and Conditions

8. Fig. 6
Prolactin (PRL) levels in culture media from epithelial (A), stromal (B), and myometrial cells (C) isolated from uteri without adenomyosis (white bars) and with adenomyosis (black bars) and under the influence of estradiol (E2), as determined by EIA. Bars represent the mean ± standard error of the mean. #P < 0.05, ###P < indicate statistically significant differences between untreated and treated cells within each experimental group (control or adenomyotic); *P < 0.05, **P < 0.01 indicate differences between normal uterine and adenomyotic cells within the untreated or E2-treated group. Statistically significant differences were determined using 2-way analysis of variance, followed by Bonferroni's posthoc test.
Domestic Animal Endocrinology  , 1-13DOI: ( /j.domaniend )
Copyright © 2016 Elsevier Inc. Terms and Conditions