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Human adult endothelial cell growth in culture

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1 Human adult endothelial cell growth in culture
Bruce Jarrell, M.D., Elliot Levine, Ph.D., Sandor Shapiro, M.D., Stuart Williams, M.D., R.Anthony Carabasi, M.D., Stephen Mueller, Ph.D., Susan Thornton, Ph.D.  Journal of Vascular Surgery  Volume 1, Issue 6, Pages (November 1984) DOI: / (84) Copyright © 1984 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

2 Fig. 1 A, Detection of factor VIII—related antigen by indirect immunofluorescence in HAECs. Human endothelial cultures were grown to confluency, extracted with acetone, and incubated with rabbit anti-human factor VIII—related antigen, 1:100 dilution, followed by goat anti-rabbit IgG-FITC (1:100). B, As control culture, smooth muscle cells cultured from the same vessel were treated similarly. The human adult endothelial cells stain intensely for factor VIII—related antigen. (Original magnification, × 1260.) Journal of Vascular Surgery 1984 1, DOI: ( / (84) ) Copyright © 1984 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

3 Fig. 2 Phase-contrast photomicrographs of confluent early passage endothelial cells derived from adult human arteries and veins. Cells were cultured in medium 199 containing 20% fetal calf serum, 20 mg/ml ECGF, and 90 mg/ml heparin. Donor vessels included the following: A, Abdominal aorta HAA 2/7, CPDL 11. B, Femoral artery HFA 2/18, CPDL 7. C, Iliac vein HIV 2/18, CPDL 7. D, Superior mesenteric artery HMA 2/18, CPDL 7. E, Pulmonary artery HPA 2/18, CPDL 17. (Original magnification, ×266.) Journal of Vascular Surgery 1984 1, DOI: ( / (84) ) Copyright © 1984 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

4 Fig. 3 Growth curves for adult human endothelial cell lines.
Journal of Vascular Surgery 1984 1, DOI: ( / (84) ) Copyright © 1984 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

5 Fig. 4 Total cell yield for adult human iliac vein endothelial cells in culture. Journal of Vascular Surgery 1984 1, DOI: ( / (84) ) Copyright © 1984 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

6 Fig. 5 Proliferative life spans of HAEC lines.
Journal of Vascular Surgery 1984 1, DOI: ( / (84) ) Copyright © 1984 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions


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