1. Bioinformatics Resources Provided by WV-INBRE
Mary E. Davis, Ph.D.
Bioinformatics Core Director

2. Outline Overview of WV-INBRE Technology supported
Agilent microarray
Illumina HiSeq1000 (Next Generation Sequencing)
Bioinformatics analysis resources supported
Ingenuity Pathways Analysis (IPA)
Partek Genomics Suite
(Galaxy)

5. Technology Microarray Next Generation Sequencing
Agilent (Marshall Univ—WV-INBRE)
Affymetrix (WVU Neuroscience CoBRE)
Next Generation Sequencing
Illumina HiSeq (Marshall Univ—WV-INBRE)
Illumina MiSeq (WVU Neuroscience CoBRE)

6. Bioinformatics Tools: Ingenuity

8. IPA Function Result

9. IPA: Effects on Function

10. IPA: Upstream Regulators

11. IPA: Canonical Pathways

13. IPA: Gene Annotation

14. Example of Findings in Gene Annotation
Ingenuity scientists read literature and enter results using a structured vocabulary. This, in combination with disease and function ontologies, is basis for relationships in pathways.

15. IPA: Pathways