1. and its Interference Effects Upon Abbott Aeroset/Architect Assays
Lipaemia
and its Interference Effects Upon
Abbott Aeroset/Architect Assays
Stuart Pople
University Hospital of Wales
Cardiff

2. L- index 5 – 10 lipaemic > 10 grossly lipaemic
tests may be susceptible to interference

3. IntraLipid – fat emulsion
soya bean oil, egg yolk, glycerin
200 – 600nm diameter
Native lipaemia – variable mixture of macro molecular lipid-protein complexes
nm diameter

4. Method infranatant Aeroset 20 Endogenous lipaemia serum/plasma mixed
ultracentrifuged
g, 3 mins.
(max. 24 hr. delay)
infranatant
Aeroset Endogenous lipaemia
6 TPN

5. Data Analysis
CLIA-88
Clinical Laboratories Improvements Amendments U.S. statutory regulatory body setting proficiency testing standards for all aspects of laboratory medicine.
Publish criteria for most analytes:
Total Allowable Error TEa
Factor in: analytical imprecision and bias,
biological variability,
medical decision intervals,
impact on clinical interpretation etc.

6. Anal’ TEa Na 4 mmol/l Alb 3.5 g/l Uric 0.06mmol/l K 0.5 mmol/l TP
CRP
? mg/l Cl
mmol/l
Phos
0.14 mmol/l
Amy
IU/l
CO2
4 mmol/l
ALP
IU/l CK
IU/l
Urea
mmol/l
AST
IU/l
GGT
IU/l
Creat
umol/l
T.Bili
umol/l
LDH
IU/l
Gluc
mmol/l
D.Bili
ALT
IU/l Ca
0.25 mmol/l Mg
0.2 mmol/l

7. Assays Demonstrating No Significant Interference
Assays where paired results fall within TEa, at
lipaemia levels up to 100 :-
Potassium AST
Bicarbonate ALP
Urea GGT
Creatinine CK
Calcium Amylase
Albumin (BCG) LDH (L P)
CRP Direct Bilirubin

15. Analytes Demonstrating Significant Lipaemia Interference
L-index (>TEa)
Direction
Sodium 30
Neg
Chloride 50
Glucose 70
Phosphate
10 endo, TPN
T. Bilirubin
? Variable
sl. Neg
Magnesium 20
Pos
ALT 15
Total Protein 10
Uric Acid
10 Endo’ (no int’ with TPN)