1. Relative FOXM1and Met protein levels (normalized to GAPDH)
Supplementary Figure S1
(New)
Relative FOXM1and Met protein levels (normalized to GAPDH) B M C pF
FOXM1
Met A Si
siCtr (nmol)
siFOXM1 (nmol)
100 50
GAPDH
BxPC-3
pCtr (mg)
pFOXM1 (mg) 2 1
Colo357
Fold
1.0
1.1
5.3
8.9
2.6
4.1
1.2
0.4
0.1
0.3
“M”, Mock; “C”, SiControl; “Si”, SiFOXM1
“M”, Mock; “C”, Control vector; “pF”, pFOXM1

2. A B C Supplementary Figure S2 (New) Relative Met promoter DNA
(%, normalized to Input)
(Fold, normalized to Input)
#2 and #1 : 5’-TTATTTAATCAGCCTGCTTCCGGCTATG-3’ (-816 to -788)
#3: 5’-AAAGCTCTTTTTTAAAAATCATCATAAG-3’ (-905 to -877)
pLuc-Met-580 +1
Luc
+236
-344
pLuc-Met-1069
-833
#2 #1
pLuc-Met-1259
-1023
#2 #1 #3 A
Relatvie promoter
activity (%)
activity (fold) C FG
PANC-1
Putative FOXM1-binding sites on Met promoter

3. Relative levels (normalized to GAPDH)
Supplementary Figure S3
(New)
Relative levels (normalized to GAPDH) FG
AsPC-1

4. A B Supplementary Figure S4 (New) AsPC-1 FG FOXM1 p-Met pAKT GAPDH
pERK1/2
pSTAT3
AsPC-1 FG
Met
siControl
HGF+siControl
HGF+siMet#1
HGF+siMet#2
Control
HGF
HGF+PHA
ERK1/2
AKT
STAT3 A B
Fold
1.0
4.0
1.2
0.4
4.3
0.3
2.8
0.2
8.5
2.2
3.1
5.3
2.1
1.1
7.5
1.8
0..
8.9
5.1
1.6
6.5
2.7
4.1
0.8
6.9
2.5
1.5
3.8
2.6
3.4
2.3
2.9

5. FG Relative promoter activity (fold)
Supplementary Figure S5
(New) FG
Relative promoter
activity (fold)
0.2
0.01
0.6
Cyclin B1-Luc (mg)
pRL (mg)
pCtr (mg)
pFOXM1 (mg)
HGF (ng/ml) - 20
PHA (mM)
0.5
P < 0.001
P = 0.001
PANC-1

6. Relative protein levels (normalized to GAPDH)
Supplementary Figure S6
(New)
Relative protein levels (normalized to GAPDH) FG
PANC-1

7. FG PANC-1 Supplementary Figure S7 Relatvie promoter activity (fold)
FOXM1-Luc(mg)
0.2
0.2
0.2
0.2
0.2
pRL(mg)
0.01
0.01
0.01
0.01
0.01
pFOXM1(mg)
0.6
0.6
0.6
0.6
0.6
HGF (ng/ml) - 20 20 20 20
FR (mM) - - 10 - -
GSK690693(mM) - - - 10 -
S3I-201 (mM) - - - -
100
PANC-1
P = 0.004
P = 0.002
P < 0.001
P < 0.001
Relavtive promoter
activity (fold)
FOXM1-Luc(mg)
0.2
0.2
0.2
0.2
0.2
pRL(mg)
0.01
0.01
0.01
0.01
0.01
pFOXM1(mg)
0.6
0.6
0.6
0.6
0.6
HGF (ng/ml) - 20 20 20 20
FR (mM) - - 10 - -
GSK690693(mM) - - - 10 -
S3I-201 (mM) - - - -
100

8. Supplementary Figure S8
Control
HGF
HGF +
siFOXM1
PHA665752
P = 0.002
Colony number
P = 0.777
P < 0.001
HGF + siFOXM1
HGF + PHA665752

9. A B FG Supplementary Figure S9 PANC-1 Tumor weight (g) FOXM1 Met Mock
Control
pFOXM
PANC-1
FOXM1
Met FG
pFOXM1
siFOXM1 A
siFOXM B
P < 0.001
Tumor weight (g)
P = 0.014

10. A B C D E F Supplementary Figure S10 (New)
Relative protein levels (normalized to GAPDH)
Relative protein levels (normalized to GAPDH) C D
Relative protein levels (normalized to GAPDH)
Relative protein levels (normalized to GAPDH) E F
Relative protein levels (normalized to GAPDH)
Relative protein levels (normalized to GAPDH)