1. Article category: Notes
Field under which the paper is to be reviewed: Molecular biology
Identification of a novel Arabidopsis mutant showing sensitivity to histone deacetylase inhibitors
Nguyen Hoai Nguyen1 • Chan Young Jeong1, 2 • Won Je Lee1, 2 • Hojoung Lee1, 2, *
1Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul , Republic of Korea.
2Institute of Life Science and Natural Resources, Korea University, Seoul , Republic of Korea.
*Corresponding author:
Hojoung Lee
Address: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul , Republic of Korea.
Telephone:
Fax:

2. Supplementary materials

3. Figure S1 Col-0 shdi1 Col-0 shdi1 Control Control
NaB 200 µM + IAA 5 µM
TSA 5 µM + IAA 5 µM
Figure S1. Growth of the shdi1 mutant on screening media.
Four-day-old seedlings of shdi1 and Col-0 were transferred to the indicated media for 2 weeks.

4. Recombination frequency (%)
Figure S2
(A)
No. of F2 seedlings
Ratio WT
shdi1
shdi1 was crossed
with Col-0
163 51 3 1
with Ler
164 53
Table 1. Segregation of nia6 phenotype in F2
(B)
SSLP markers on Chr. 5
Recombination frequency (%)
nga225 50
AthEMC
36.6
nga249
nga151 40
nga106
41.6
nga76
Figure S2. The shdi1 is a recessive mutant.
(A) The shdi1 mutant was crossed with wild-type plants (Col-0 and Landsberg erecta), and the F2 progeny were sown on screening media to test the recessiveness of shdi1 phenotype. (B) For mapping, shdi1 was crossed with the Landsberg erecta ecotype. The location of the shdi1 locus was mapped on the basis of shdi1 phenotypes with several SSLP markers.

5. Figure S3 (A) (B) Light Dark Col-0 shdi1 * Col-0 shdi1
Hypocotyl length (mm)
(A)
Col shdi1
Dark
(B)
Figure S3. The shdi1 mutant showed a shorter hypocotyl than wild-type plants.
(A) Seeds of shdi1 and Col-0 were put on 1/2× MS medium and grown in light conditions for 2 weeks. (B) Seeds of shdi1 and Col-0 were put on 1/2× MS medium and grown in dark conditions for 4 days. The asterisks above the columns indicate significant differences in hypocotyl length between shdi1 and Col-0 (P < 0.05).

6. Table S1. Primers used For RT-PCR Name Forward Primer (5’-3’)
Reverse Primer (5’-3’)
ACTIN8
ATGAAGATTAAGGTCGTGGCA
TCCGAGTTTGAAGAGGCTAC
AS1
AGACAGTTCGGTCCGAGAGA
GGGAACCTCAGCAGCAATCT
AS2
CCTCAACGAGCTTCACCCTT
GATAGTGGTCTCTCACGGCG
KNAT1
TGACAACAGCACCACTCCTC
TTGCTTCCTGAATGGGCCTT
PIF4
ATCGGGGATTAGGGAAACAG
ATCCTGATCGTTGGTTCGAC
IAA17
AAGATCCAGCCAAACCTCCG
TGGCAGGAAACCATCACGTT
MAX2
TCGACGGTGTGGCTTTATGT
AAGCGAGAGACTCCTCTGGT
BRC1
GTCTCATATCCGACCGGTGT
TGGCTGCGATGGTGGTTAAT
DWF4
TGCCGGACATGAGACTTCTT
GTTGTTTCCCCACGTCGAAA
UBC21
TTACGAAGGCGGTGTTTTTC
TGAACCCTCTCACATCACCA