1. Colonic expression of DRA (downregulated in adenoma) mRNA in HLA-B27/β2M rat compared with normal control animal, as well as in patients with ulcerative colitis.A: Northern blots of total RNA isolated from the cecum (C) and proximal (P) and distal (D) colon...
Colonic expression of DRA (downregulated in adenoma) mRNA in HLA-B27/β2M rat compared with normal control animal, as well as in patients with ulcerative colitis.A: Northern blots of total RNA isolated from the cecum (C) and proximal (P) and distal (D) colon of both HLA-B27/β2M transgenic rat and normal control animal were hybridized sequentially to probes for DRA and villin. Ethidium bromide (EtBr)-stained Northern gel is shown below autoradiographs.B: after normalization to villin expression, fold-reduction of DRA mRNA expression in each colonic segment in transgenic rats compared with control animal was determined.C: Northern blot of DRA using RNA isolated from 3 patients with ulcerative colitis. Paired samples of tissue were obtained from each patient, one from area of active colitis (UC) and one from region with normal histology (N). Blot was reprobed for 18S RNA, and abundance of DRA mRNA, normalized to that of 18S, was quantitated and depicted at bottom.
Hongyun Yang et al. Am J Physiol Gastrointest Liver Physiol 1998;275:G1445-G1453
©1998 by American Physiological Society