1. Positive signals of MGIT cultures in the BACTEC 960 system
Mycobacterial DNA extraction
Grouping of rpoB DPRA
Subspecies identification by hsp65 PRA
MTB (235 bp )
8 groups NTM (136 bp )
Concordant results
Disconcordant results
16S r DNA sequencing and Conventional biochemical methods
Subspecies identification report
Final subspecies identification report
Figure 1. An flow chart of the identification of Mycobacterial species from clinical specimens by combined rpoB duplex PCR (DPCR) and hsp65 PCR-Restriction Fragment Length Polymorphism analysis (PRA)