1. BIO-SAFETY CABINET; A TOOL FOR INFECTION CONTROL IN HEALTH CARE FACILITIES
HOTEL WATERBUCK-NAKURU,KENYA.
13-15TH NOV.2013
PRESENTED BY
OMONDI GEORGE OTIENO
BIO-MED AND BIO-SAFETY CABINETS CERTIFIER.
KEMRI/CDC PROGRAM.
Cell phone;

2. BACKGROUND/INTRODUCTION
The increasing need to work with TB and other airborne infectious agents places greater reliance upon the following containments offered by a BSC;
Personnel (2). Product (3). Environment
Proper risk assessment, selection, installation, service ,certification and use of BSC play a pivotal role in bio-containment programme, thus minimizes infections and contaminations in medical/research laboratory set-ups.

3. WHAT IS A BSC?
A biological Safety cabinet is a ventilated box/enclosure which uses directional air flow and HEPA Filters to provide the following protections;
BSCs serve as primary containment devices for operations involving potential splashes, spills or aerosolization of hazardous biological materials.

4. ELEMENTS OF CONTAINMENT
A section of the lab brought down for a BSC installation in a District Hospital in Kenya. This shows no proper assessment was carried out prior to BSC purchase.
Proper mix of the three elements is assessed by needs and specific hazards of any facility

5. A well maintained & located BSC will operate smoothly to give the required protections

6. BSC CERTIFICATION It involves testing for; Site installation test
Certification is the testing process used to verify proper continuous functionality of BSCs using duly calibrated equipment by a qualified certifier.
Below is a Kenyan certifier testing for down flow velocity in a class IIA2 BSC.
It involves testing for;
Site installation test
Down flow velocity test
Inflow velocity test
HEPA Filter leak test
Air flow smoke pattern test
Cabinet integrity test( for class AI cabinets only)

7. WHEN DO WE CERTIFY BSCs? Consider the following;
Equipment used must be calibrated, they include;
When installed for first use
Annually
When relocated
When major repairs are done/ change of HEPA filters
Thermal anemometer/hot wire
Aerosol photometer
Aerosol generator
Direct instrument measurement (DIM)
Smoke pattern testing equipment

8. A SECTION OF TEST RESULTS/ REPORT
Allowable Ranges
Initial measurements
Final Setpoints
HEPA Filter test
PROGRAM / SPONSOR
Cabinet #
Manufacturer
Model
Serial #
Intake
Downflow
Inflow Final Setpoints
Supply
Exhaust
Smoke Test
Mag. Gauge
Cabinet location 01
Thermo
1376
58-68
103 58 62 ok
pass
N/A 
Migori DH
DGHA 02
Gelaire
BSB4
12250
0.4 m/s
70-80
0.39 72 81 77
Fails OK
Pass
125
Blood Bank Kisumu 03
ESCO
Airstream
m/s
0.37
0.28
 N/A
KMTC Kisumu 04
BH 550
Class 1 75
N/A 50
Failed
Nyamira D. Hospital 05
Nuve NA
m/s
0.81
0.38
Kericho D.Hospital 06
1285
65-75
113 67
115 69
Changed
0.55
NBTS 07
Faster
BHEN2006
382
.4 m/s
m/s
0.31
0.34
KNH 08
Nuaire
440
.46 m/s
0.38 m/s
0.2
0.3
GFC ward KNH 09
Lab Caire
SC12R
SCR-197
0.52
0.22
0.65
0.36
be changed
TB lab KNH 10
104830
108 70
TB lab 11
BHEN2004
S763
0.4
Virology lab kabete
GDD 12
S746
0.32 13
Telstar
Biostar plus
24309
0.42
0.27
manual needed
Culture/isolation Kabete 14
AC2 E41
.51 m/s
m/s
CVL Kabete 15
100927
66-76 94
106 59
HIV lab 2KEMRI 16
Baker
SG600UK
63252
61-71 93 63
105
VHF lab

9. INDICATORS AND ANALYSIS
The following indicators were looked into out of 52 BSCs
Analysis of the indicators
BSC non- functionality =6
Poor facility design = 31
BSC never been certified =47
BSC user incompetency =47
No basic maintenance = 47
Poor containment level =48

10. CHALLENGES FACED In adequate funds
Poor facility designs that led to breaking of walls thereby consuming much time in a facility
In adequate certification equipment led to sharing hence lowering the pace of work.

11. RECOMMENDATIONS AND CONCLUSION
To improve lab safety in our facilities and move towards accreditation, it is crucial that the Kenya ministry of Health;
Have a structural local BSC maintenance and certification program.
Put in place annual BSC user training.
Standardize the facility designs in Kenya
Allocate funds for BSC annual maintenance and certification exercise and spare parts replacement.

12. ANY QUESTIONS? SPECIAL ACCOLADES TO;
DIVISION OF GLOBAL HIV AIDS( DGHA)
GLOBAL DISEASE DETECTION (GDD)
KEMRI/CDC
MOH,KENYA.
EAGLESON INSTITUTE. SANFORD, MAINE, USA.
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