1. Bernard et al, Supplementary Figure S4
pTRIP-DU3-TRE-MCS
pTRE
Supplementary Figure S4. The pTRIP-DU3-TRE-MCS lentivector.
The lentivector pTRIP-DU3-TRE-MCS was derived from the self-inactivating lentivector pTRIP-DU3-EF1a-EGFP (1). The EF1a promoter was replaced by the tetracycline-inducible pTRE promoter from the vector pTRE-tight (Clontech) and a multiple cloning site (MCS) was inserted in place of the GFP gene.
The cDNAs coding D133P53a, D133P53b, or D133P53g (XhoII-BamHI fragments), respectively, were subcloned into the SalI-BamHI sites of pTRIP-DU3-TRE-MCS. The resulting lentivector plasmids were pTRIP-TRE-D133P53a, -D133P53b, and -D133P53g .
1. Sirven A, Ravet E, Charneau P, et al. Enhanced transgene expression in cord blood CD34(+)-derived hematopoietic cells, including developing T cells and NOD/SCID mouse repopulating cells, following transduction with modified trip lentiviral vectors. Mol Ther 2001;3(4):