1. Biotech Lab Safety Specifics
Abbrevieations:
PPE – Personal protective equipment
Should be chosen based on specific risks
Glasses with side protection if low splash risk
Chemical goggles if large splash risk
UV glasses with full face shield if short wave UV is used to view DNA banding
Other example: aprons, lab coats, gloves, face masks and shoe covers

2. MSDS – Material Safety Data Sheet
Lists potential hazards associated with the chemicals used in a laboratory

3. Biological Safety Level
BSE 1 – Not a specific threat to handling individual
All HS labs and most college labs
BSE 2 - Moderate threat to individuals and the environment
Some advanced college labs
BSE 3 & 4 - pose a threat too great to for use an educational environment
The amount of government regulation goes up with each level

4. General Laboratory Hazard Signs
Harmful
Explosive
Corrosive
Dangerous for the environment
Flammable
Toxic
Oxidizing
Radioactive
Biohazard
Poison
General danger
Electrical hazard

5. Disposal of Biohazardous Material
Autoclave
Uses pressure to increase the boiling point of water to 121oC
10% Bleach for countertops
Must sit for 10min to be effective
Recommended after each biological lab

6. Volumetric Equipment Most Common Graduated Flask Graduated Cylinder
Erlenmeyer Flask
Reagent Bottle

7. Pipette Types Volumetric … has graduations
Serologic … has calibrated tip
Pasteur (transfer) … made of glass & does not have graduations
Used to transfer liquid from one container to another
Disposable transfer pipette
Standard thin stemmed plastic pipette