1. T
Standard phylogenetic approaches and Three-taxon statement analysis remain as tools of discovery: Cryptic genera in Atraphaxis (Polygonaceae)
Olga V.Yurtseva1, Evgeny V.Mavrodiev2
1 Department of Higher Plants, Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory, 1-12, , Moscow, Russia,
2 Florida Museum of Natural History, University of Florida, Gainesville, Florida, U.S.A.,
Purpose
We aim to reveal relationships within Atraphaxis L. s.l. as broadly circumscribed by Tavakkoli et al. (2015) using standard phylogenetic techniques and Three-taxon statement analysis (3TA).
Methods
Maximum Likelihood analysis (ML) and Bayesian Inference (BI) of the tribe Polygoneae based on nuclear (ITS) and combined plastid (three loci) datasets; Standard Maximum Parsimony (MP) and 3TA of 27 morphological characters
Results
Distributional areas of Bactria, Persepolium and Atraphaxis
Bactria lazkovii
Atraphaxis
Bactria ovczinnikovii
Peerspolium
Nuclear phylogeny of the tribe Polygoneae (ML, BI)
Plastid phylogeny of the tribe Polygoneae (ML, BI)
Monophyly vs paraphyly of Bactria (plastid vs nuclear topology)
The aLRT support values ≥ 0.8 from ML analysis / posterior probabilities ≥ 0.9 from the BI
The aLRT support values ≥ 0.8 from ML analysis/
posterior probabilities ≥ 0.9 from the BI
Morphological phylogeny
of Atraphaxis s.l. (standard MP)
Nested most parsimonious hierarchy
of patterns (3TA, morphology)
Bactria
B. lazkovii
B. ovczinnikovii
P. salicornioides
Persepolium
Atraphaxis
A. toktogulica
A. virgata
Conclusions
The almost fully congruent topologies that result from (1.) ML and BI of the molecular datasets and (2.) from both standard MP and 3TA analyses of the morphological data elucidate the discovery of the generic rank of traditionally circumscribed Atraphaxis s.str., as well as Bactria and Persepolium, and suggest potential hybridization processes
within the tribe Polygoneae.
MP Bootstrap support values ≥ 50%

2. Atraphaxis teretifolia
Post Scriptum. New intriguing story of Atraphaxis s.str. and allies: congruencies, incongruences and new species
The best tree from ML analysis of the combined plastid dataset:
trnL intron, trnL-trnF IGS, and rpl32–trnL(UAG) IGS regions of cpDNA
Atraphaxis spinosa
(A. sect. Atraphaxis)
Atraphaxis virgata
(A. sect. Tragopyrum)
Atraphaxis teretifolia
(A. sect. Physopyrum)
The aLRT support values ≥ 0.8 from ML analysis / posterior probabilities ≥ 0.9 from the BI
Persepolium salicornioides
(Persepolium)
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