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Volume 64, Pages (November 2016)

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1 Volume 64, Pages 83-89 (November 2016)
Postictal alterations induced by intrahippocampal injection of pilocarpine in C57BL/6 mice  Isabel Vieira de Assis Lima, Alline Cristina de Campos, Paula Maria Quaglio Bellozi, Juliana Guimaraes Doria, Fabiola Mara Ribeiro, Marcio Flavio Dutra Moraes, Antonio Carlos Pinheiro de Oliveira  Epilepsy & Behavior  Volume 64, Pages (November 2016) DOI: /j.yebeh Copyright © 2016 Elsevier Inc. Terms and Conditions

2 Fig. 1 PILO-induced SE mice display cognitive impairment. Results are shown as mean±SEM. (A) Recognition index (%) of mice in the Novel Object Recognition task (n=7 for vehicle group, n=9 for PILO group; *p<0.05). (B) Freezing time (sec.) of mice in the Contextual Fear Conditioning test (n=5 for vehicle group, n=5 for PILO group, *p<0.05). Epilepsy & Behavior  , 83-89DOI: ( /j.yebeh ) Copyright © 2016 Elsevier Inc. Terms and Conditions

3 Fig. 2 PILO-induced SE mice have reduced cell viability. Results are shown as mean±SEM. (A, C, E) PILO-induced SE mice show a decrease in hippocampal (CA1, CA3, DG) cellular viability as visualized by Nissl staining (n=5 for all groups; *p<0.05; **p<0.01). (B, D, F) Representative photomicrographs of CA1, CA3, and DG regions stained with cresyl violet. Epilepsy & Behavior  , 83-89DOI: ( /j.yebeh ) Copyright © 2016 Elsevier Inc. Terms and Conditions

4 Fig. 3 PILO-induced SE mice show increased neuronal loss. Results are shown as mean±SEM. (A, C, E) NeuN staining reveals that PILO-induced SE mice present an increase in hippocampal (CA1, CA3, DG) neuronal loss (n=5 for all groups; *p<0.05; **p<0.01). (B, D, F) Representative photomicrographs of the CA1, CA3, and DG regions stained with anti-NeuN antibody. Epilepsy & Behavior  , 83-89DOI: ( /j.yebeh ) Copyright © 2016 Elsevier Inc. Terms and Conditions

5 Fig. 4 PILO-induced SE mice display increased microglial proliferation. Results are shown as mean±SEM. (A, C, E) PILO-induced SE mice display increased microglial proliferation in hippocampal CA1, CA3, and DG regions, shown by Iba-1 staining (n=3 for vehicle, n=5 for PILO; *p<0.05; ***p<0.001). (B, D, F) Representative photomicrographs of the CA1, CA3, and DG regions stained with anti-Iba-1 antibody. Epilepsy & Behavior  , 83-89DOI: ( /j.yebeh ) Copyright © 2016 Elsevier Inc. Terms and Conditions

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