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Lipidomic Profiling of Neoplastic, Pre-Neoplastic, and Healthy Tissues Using Electrocautery Paired with Mass Spectroscopic Equipment Presenter: Joseph.

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Presentation on theme: "Lipidomic Profiling of Neoplastic, Pre-Neoplastic, and Healthy Tissues Using Electrocautery Paired with Mass Spectroscopic Equipment Presenter: Joseph."— Presentation transcript:

1 Lipidomic Profiling of Neoplastic, Pre-Neoplastic, and Healthy Tissues Using Electrocautery Paired with Mass Spectroscopic Equipment Presenter: Joseph M. Gosnell Hepatocellular Carcinoma

2 Case Study 46 YO Male Diagnosed with NAFLDNASH progression after presenting with hematemesis, esophageal varices, splenomegaly, and diffuse hepatic fibrosis After three years, patient developed hepatocellular carcinoma (HCC), a solid liver tumor, manifesting in three distinct locations less than 3cm in diameter It was decided that the patient would undergo a TIPS procedure to reduce hepatic portal vein hypertension, allowing for a chemoembolization of the HCC masses in the liver Patient decompensated when the procedure formed a thrombus which traveled to a branch of the R marginal artery Patient continued to decompensate, resulting in hepatorenal syndrome in December, and multiple organ failure in March/April of this year. Patient died at 50 YO on April 6, 2015.

3 Resection of Hepatocellular Carcinoma Tumors
Two Surgical Approaches Anatomical Resection Lobectomy Segmentectomy Subsegmentectomy Wedge Resection

4 Electrocautery A small metal probe is electrified to either cut through tissue or cauterize (burn shut) blood vessels. Also used to resect neoplastic tissues, i.e. tumors.

5 Mass Spectroscopy Several methods used to identify tissue intra-operatively. REIMS: Rapid Evaporative Ionization Mass Spectroscopy TSI-MS: Tissue Spray Ionization Mass Spectroscopy

6 Phospholipids of Interest
Phospholipid: cell membrane components with lipids and phosphate groups Differences in phospholipid content between neoplastic tissue and healthy tissue can indicate differences between tumors, pre-tumors, and healthy tissue. PA PI PG PE PS

7 Differences between Neoplasms and Healthy Tissue

8 Recent Pairing Efforts
Waters Corporation iKnife System: pairs electrocautery with REIMS equipment for intraoperative identification of neoplastic, pre-neoplastic, and healthy tissue Who will develop the next system with marketability? Liberty University?

9 Applications: From Surgical Oncology to Food Testing
This technological pairing is applicable to more than surgery. Research Rapid analysis of phospholipids from organisms and tissue samples Could reduce the amount of workflow necessary for analysis Food Services and Verification Can be used by FDA officials to test foods for pathogenic organisms Can be used by restaurants and restauranteurs to determine meat quality (i.e. GrouperTilefish, Halibut, King Salmon, etc.)

10 References Balog, J., Sasi-Szabo, L., Kinross, J., Lewis, M.R., Muirhead, L.J., Veselkov, K., Mirnezami, R., Dezso, B., Damjanovich, L., Darzi, A., Nicholson, J.K., and Takats, Z. (2013). Intraoperative tissue identification using rapid evaporative ionization mass spectrometry. Science Translational Medicine 5(194), p Chau, G.Y. (2014). Resection of hepatitis-B virus-related hepatocellular carcinoma: Evolving strategies and emerging therapies to improve outcome. World Journal of Gastroenterology, 20(35), p MedlinePlus. (2015). Electrocautery. neplus/ency/article/ htm Waters Corporation. (2015). iKnife system. s/en_US/REIMS-Research- System-with-iKnife-Sampling- Device/nav.htm?cid= &locale=en_us Waters Corp. iKnife. (2015). ch?v=Z9yVeMba0m0 Wei, Y., Chen, L., Zhou, W., Chingin, K., Ouyang, Y., Zhu, T., Wen, H., Ding, J., Xu, J., and Chen, H. (2015). Tissue spray ionization mass spectrometry for rapid recognition of human lung squamous cell carcinoma. Scientific Reports, 5, 10077; doi: /srep10077.

11 Picture Credits Hepatic Lobectomy. rg/liver_tumor_center/treatme nts/surgery_remove_tumor.htm l Grouper. assive-goliath-grouper- snatches-shark-single- bite/story?id= Hepatic Segmentectomy. rg/liver_tumor_center/treatme nts/surgery_remove_tumor.htm l Hepatic Wedge Resection. rg/liver_tumor_center/treatme nts/surgery_remove_tumor.htm l Hepatocellular Carcinoma. m/category/hepatocellular- carcinoma/hepatocellular- carcinoma-hepatocellular- carcinoma M. Gosnell. es/flint/obituary.aspx?pid= Monopolar Cautery. m/medical_exhibits.php?exhibi t=13263_03X&query=monopola r%20cautery%20bipolar%20elec tric%20current%20electrode%2 0vaporization Phosphatidic Acid. dex.php?option=com_content &view=article&id=389&Itemid= 259&catnumber=841188 Phosphatidylethanolamine. dex.php?option=com_content &view=article&id=389&Itemid= 259&catnumber=841188 Phosphatidylglycerol. dex.php?option=com_content &view=article&id=389&Itemid= 259&catnumber=841188 Phosphatidylinositol. dex.php?option=com_content &view=article&id=389&Itemid= 259&catnumber=841188 Phosphatidylserine. dex.php?option=com_content &view=article&id=389&Itemid= 259&catnumber=841188

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