1. Methods for site-directed mutagenesis
Methods for site-directed mutagenesis. A) Insertion of a new sequence at the site of action of a restriction enzyme by ligating a small oligonucleotide sequence within the reading frame of a gene. B) Use of a primer sequence that is synthesized to contain a mismatch at the desired site of mutagenesis. C) Outline of the PCR-based methodology. (From
Source: Methods of Molecular Analysis, The Basic Science of Oncology, 5e
Citation: Tannock IF, Hill RP, Bristow RG, Harrington L. The Basic Science of Oncology, 5e; 2016 Available at: Accessed: October 16, 2017
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