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Hybridization.

Published byColleen Reed Modified over 6 years ago

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Presentation on theme: "Hybridization."— Presentation transcript:

1 Hybridization

2 Reverse transcription

3 Complementary DNA

4 cDNA library

5 Polymerase chain reaction

6

7 Real time PCR

8 DNA chip

9 Direct seeding Robot spotting InkJet technology – HP/Canon

10 Photolithography - Affymetrix

11 1,1 dimethoxytrityl

12 GeneChip® System Probe Arrays (chips) Fluidics Station Software
Scanner Software Data Analysis

13 The Dimensions of a GeneChip
* 5” 5” 5µm Millions of identical probes / feature Up to ~6,500,000 features / chip 1.28cm 1.28cm

14

15

16

17

18 Photolithography - Febit/NimbleGen

19 Nanogen biochip

20 DNA sequencing

21 Restriction Fragment Length Polymorphism

22 Sensing methods in biochips

23 Take home message Library: genomic DNA vs cDNA hybridization

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