1. From: Topography of Lymphatic Markers in Human Iris and Ciliary Body
Invest. Ophthalmol. Vis. Sci ;56(8): doi: /iovs
Figure Legend:
Ciliary body. (A) PDPN immunoreactivity was detected at the anterior margin of the ciliary muscle (α-SMA, blue) in the area of the trabeculum ciliare (asterisk). Here, an association with blood-vessels (CD31, green) was not obvious. More posteriorly, between muscle fibers of the ciliary body, CD31-positive blood vessels (green) were detectable, but podoplanin positive vessels were absent. (B) Occasionally, single PDPN-immunoreactive cells (red, arrowhead) were detectable in the connective tissue between muscle fibers (α-SMA, blue). These cells were not associated with CD-31 immunoreactive blood vessels (green). (C) Interspersed in the ciliary muscle, LYVE-1–positive cells (green) were detectable (arrowheads), these cells were PDPN-negative. Further, a PDPN immunoreactive signal was occasionally detectable on the margin of a cell-free lumen that appeared between bundles of muscle fibers (arrows). These observed immunoreactive signals were not colocalized with LYVE-1. (D) Similar podoplanin immunoreactive signals (red, arrows) were also detected surrounding cell-filled lumina. Again, these immunoreactive signals were not colocalized with LYVE-1 (green). Note a single LYVE-1+/PDPN− cell adjacent to the podoplanin-positive lumen (arrowhead). (E) Podoplanin-immunoreactive signals (red, arrows) were not associated with PROX-1 (green)-positive nuclei. (F) In double-labeling experiments applying VEGFR3 (red) and α-SMA (green), VEGFR3-immunoreactive cells were detectable anteriorly of the ciliary muscle (asterisk). (G) A VEGFR3-immunoreactive signal (red) was also detectable in the ciliary muscle, while any PROX-1–positive structures (green) were absent within muscle fibers as well as interspersed connective tissue fibers (green color in this figure represents background fluorescence). Note the VEGFR3-immunoreactive structures (arrowheads), most likely representing blood vessels, as detected in iris. (H) High-resolution images of the ciliary body revealed cell-free lumina bordered by a VEFGR-1–immunoreactive cells (red). These cells did not show a nuclear immunoreactivity for PROX-1 (green) or CCL21-immunoreactivity (blue). (I) VEGFR3-immunoreactive lumina (red, arrowheads) were also colocalized with CD31 (blue), and were detected in and between muscle fibers of the ciliary body (green, α-SMA), thus representing blood vessels. Note that also VEGFR3-immunoreactive cells are also present within the ciliary body (arrows). (J) VEGFR3-immunoreactive ciliary muscle fibers (green) are colocalized with CCL21 (red), resulting in yellow-mixed color. Note VEGFR3-immunoreactive blood vessels interspersed between the muscle fibers. (K) Very rarely, CCL21 immunoreactive cells (red, arrowhead) were detectable within the ciliary body. These cells were not colocalized with VEGFR3 (green). Yellow-mixed color here (open arrowhead) corresponds to autofluorescent collagen fibers. (L) Very rarely, LYVE-1–immunoreactive cells (red) were observed displaying a FOXC2-immunoreactive nucleus (blue, arrowhead), but lacking PROX1 (green).
Date of download: 10/14/2017
The Association for Research in Vision and Ophthalmology Copyright © All rights reserved.