1. BIOMARKERS for Oxidative Stress in Veterinary Medicine, Part 2
W. Jean Dodds, DVM & Denis M. Callewaert, PhD AHVMA 2016 Columbus, OH

2. Oxford Biomedical Research & Hemopet Study
3 groups of 20 Hemopet greyhound dogs each
Normal dry dog food diet
Low-fat light kibble diet
Home made custom diet for food intolerant dogs
Samples of whole blood, serum, and saliva collected at day 0, then at 2 weeks, 1 month and 2 months
Measure catalase and/or superoxide dismutase in blood, as markers for transcription factor Nrf2 (nuclear factor 2 p45-related factor) activation rather than glutathione
Measure interleukin – 6; tumor necrosis factor –alpha, and 15F2t-isoprostane (oxidation product of arachidonic acid)
Measure TBARS : specific for malonaldehyde

3. Dietary Factors Known to Induce Nrf2
Turmeric, R-α-Lipoic acid, tert-butylhydroquinone, sulforaphane and resveratrol
Cause increases in several enzymes (including catalase and superoxide dismutase)