1. Scaling Up Of Monolayer Cells

2. Topics Covered… Introduction Definition Enlisted methods
Beadbed Reactor
Types of vessels
Roux bottle
Roller bottle
Multitray unit
Synthetic Hollow Fiber Cartilage
Opticell culture system
Plastic film
Heli-cell vessels

3. Introduction Properties of monolayer cells :
Needs charged substrate for attachment
Charge provided via
Forms monolayer
Controlled by contact inhibition
Example : all vertebrate cells
Electric ion discharge
g radiation

4. Definition of Scaling Up
Large scale production of desired compounds
Desired compounds include :
Aims : increased surface area
Hormones
Vaccines
Insulin
Interferon
Clotting 9,etc

5. Beadbed Reactor

6. Vessels used Roux Bottle Roller Bottle Commonly used in laboratory
Small portion available
S.A provided = cm3
Space occupied = cm3
Stationary vessel
Roller Bottle
Limited space for scaling up
All S.A available
Rolling motion
Modifications
Spira-cell
Glass tube
Extended S.A roller bottle

8. Multitray Unit Synthetic Hollow Fiber System Possess 10 chambers.
Interconnecting channels for media supply.
Multitasking unit.
S.A = 600cm3/chamber
Volume of unit = 12.5ltr
Disposable polystrene unit.
Efficient alike plastic flasks.
Synthetic Hollow Fiber System
Fiber enclosed in sealed cartirdge
Capillary fibers of acrylic polymer
Diameter = 350mm
Media perfused via fiber
S.A = 30cm3/ml of media volume
Suitable if polysulphone type fiber used

9. Opticell Culture System Plastic Film
Cylindrical ceramide cartridge.
Perfusion loop as a reservoir for control.
Channels of 1mm2 run through the length
S.A = 40cm3/ml of media
Suitable for –
Virus
Cell surface antigen
Monoclonal antibody production
Suspension & monolayer cell cultures
Plastic Film
Teflon (fluoroethylene propylene polymer)
Biologically inert & highly permeable
Size of bags = 5*30cm
Attachment at inside surface
Teflon tubes are wrapped round a reel with spacer
Media is pumped into bag through the tube
Growth is observed at inside surface

10. Heli-cell Vessels Packed with polystyrene ribbons
Dimension of ribbon = 3mm*5-10mm*100mm
Ribbons twisted in helical shape
Ribbons ensures good circulation of media
Cell adhere to ribbon surface