1. ExTRacting DNA from C. elegans
By: Brianna N. Kaylor,
Joseph H. Lackey
And Ryan Kelly

2. C. elegans 1,582 base pairs for C. elegans organic anion transporter
Intestine
Interested in long skinny blue intestine
Grow a lot of worms
Extract their DNA
Looking for the transporter (organic anion transporter)

3. Extracting the DNA Lyse C. elegans Bind DNA to Column Wash
Elute DNA from Column
Detect DNA
Extract
DNA
Spectroscopic quantification
Agarose gel electrophoresis detection
C. elegans
In order to extract the worms to obtain the DNA, you must first wash the dish with a M9 buffer to gather as much worm as possible. Then transfer the worms to a 15mL centrifuge test tube so you can push all the worms to a pellet at the bottom. Then extract the top layer to only have worms in the 15mL tube.

4. Techniques Gel Electrophoresis Spectrophotometer
Gel electrophoresis apparatus:
Black is negative
Red is positive
Hooks up to power supply which pulls the DNA down the gel to the positive cation
The smaller DNA will go farther down the gel
Nano dropper:
Can analyze a 1 μL sample of DNA or RNA
Gives a graph and a concentration percentage
Can analyze a 1 μL sample of DNA
Use an electromagnetic field to pull the DNA from the wells to create the bands that we can see on the UV imager.

5. Results
A260/A280 = 1.59
338 ng/nL
Discovered how to use imaging and what to look for.
How to make buffer
Use gel
Understanding how to use equipment
Understand how ladders and DNA run through pores in gel

6. Agarose gel electrophoresis
Analyzing the gel
Agarose gel electrophoresis
Poor separation of
DNA molecules > 50 kb UV UV
ethidium bromide dye is used to stain DNA

7. Results
A single band stuck in the wells is whole, uncut genomic DNA. A smear of DNA in the lane represents large pieces of partially degraded genomic DNA, Solutions: 1)Obtain greater purity of DNA 2) Use lower agarose concentrations 3) Use enzymatic digestion to cut fragments into smaller pieces
Marker
DNA
Marker
DNA

8. Acknowledgements Academy of Science program Dr. Cecile
A.R. Smith Department of Chemistry
Dr. Tashakkori
Create agarose gel
Run real dna
Test different concentrations of the same dna by using dyes and H2O
Set up electrophoresis
Dna is negatively charged
Run Red