1. iGEM Meeting – 19/04/17: SELEX & Riboswitches

2. SELEX Systematic Evolution of Ligands by EXponential enrichment
Generation of RNA (or DNA) aptamers by in vitro selection
Random DNA oligos
RNA oligos
In vitro transcription
Incubate library with ligand
In vitro transcription
Remove bound aptamers
Remove unbound aptamers
RT-PCR
Incubate library with undesired ligands
In vitro transcription
RT-PCR

3. Aptamer to Riboswitch In vivo riboswitch selection
Identification of RNA aptamers with riboswitching properties. DOI:

4. Modelling for in vivo Selection
Determine riboswitches which fold correctly
Uses free energy calculations
Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. DOI:

5. Ribozymes
In vitro selection of allosteric ribozymes that sense the bacterial second messenger c-di-GMP. DOI: / _15.

6. Toehold Switches
Toehold Switches: De-Novo-Designed Regulators of Gene Expression. DOI:

7. Dual-Colour Riboswitch
Riboswitch-Based Reversible Dual Color Sensor. DOI: /acssynbio.6b00199