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Date of download: 10/10/2017 Copyright © ASME. All rights reserved.

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1 Date of download: 10/10/2017 Copyright © ASME. All rights reserved. From: Effect of Hydroxyapatite Nanoparticles on Biotransport Phenomena in Freezing HeLa Cells J. Nanotechnol. Eng. Med. 2014;5(4): doi: / Figure Legend: The radius of particles with regard to the weight ratio of Na6(PO3)6 to HA. Each value was automatically averaged (n  > 10) and calculated by the machine software according to the preset property of the solution (1 × PBS) and particles (assumed to be circular).

2 Date of download: 10/10/2017 Copyright © ASME. All rights reserved. From: Effect of Hydroxyapatite Nanoparticles on Biotransport Phenomena in Freezing HeLa Cells J. Nanotechnol. Eng. Med. 2014;5(4): doi: / Figure Legend: Sedimentation of nanoparticle suspensions at different time points. Arrows indicate the aggregation of the HA nanoparticles. 0 h, 0.5 h, 1 h, 1.5 h, and 2 h.

3 Date of download: 10/10/2017 Copyright © ASME. All rights reserved. From: Effect of Hydroxyapatite Nanoparticles on Biotransport Phenomena in Freezing HeLa Cells J. Nanotechnol. Eng. Med. 2014;5(4): doi: / Figure Legend: Volumetric responses of HeLa cells at various temperatures during freezing at 15 °C min−1. (a) Cells in 1 × PBS, (b) cells in 1 × PBS with 0.05% HA nanoparticle suspensions without incubation, and (c) cells in 0.1% HA nanoparticles solutions without incubation.

4 Date of download: 10/10/2017 Copyright © ASME. All rights reserved. From: Effect of Hydroxyapatite Nanoparticles on Biotransport Phenomena in Freezing HeLa Cells J. Nanotechnol. Eng. Med. 2014;5(4): doi: / Figure Legend: Results obtained from individual curve fitting. (a) Representative normalized cell volume data (circles) with their individual fitting curves (solid lines) and (b) transmembrane water permeability parameters obtained from individual curve fitting and related statistical analysis results. Error bar: SD.

5 Date of download: 10/10/2017 Copyright © ASME. All rights reserved. From: Effect of Hydroxyapatite Nanoparticles on Biotransport Phenomena in Freezing HeLa Cells J. Nanotechnol. Eng. Med. 2014;5(4): doi: / Figure Legend: Results obtained from combined curve fitting. (a) Normalized cell volume at different HA concentrations with and without incubation (symbols). Solid lines show the combined fitting curves. Error bar indicates the standard error of mean. (b) Transmembrane water permeability parameters. (c) Arrhenius plot of water permeability of HeLa cells membrane (Lp) at different HA concentrations with and without incubation.

6 Date of download: 10/10/2017 Copyright © ASME. All rights reserved. From: Effect of Hydroxyapatite Nanoparticles on Biotransport Phenomena in Freezing HeLa Cells J. Nanotechnol. Eng. Med. 2014;5(4): doi: / Figure Legend: Predicted subzero water transport of HeLa cells cooled at 10, 30, 60, and 100 °C min−1. Here Vb is set as 0.35 V0.

7 Date of download: 10/10/2017 Copyright © ASME. All rights reserved. From: Effect of Hydroxyapatite Nanoparticles on Biotransport Phenomena in Freezing HeLa Cells J. Nanotechnol. Eng. Med. 2014;5(4): doi: / Figure Legend: SEM images of HeLa cells in 1 × PBS with 0–0.1% HA nanoparticles either with or without incubation at 37 °C. Arrows indicate the HA nanoparticles clusters on the cell membrane. (a) 1 × PBS, (b) 1 × PBS/0.05% HA, (c) 1 × PBS/0.1% HA, (d) 1 × PBS/0.05% HA/incubation, and (e) 1 × PBS/0.1% HA/incubation.


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