1. Introduction to the Eukaryotic Cell
Exercise 3
Introduction to the Eukaryotic Cell

2. Announcements
Students have time to observe Week 2 plates and finish their LNAs.
Post Lab 3/Pre Lab 4 are due by the time your lab meets next.
LNA Eukaryotic Cell is due next week a the start of lab.

3. Goals To learn the basic structures associated with eukaryotic cells.
To identify the differences between animal and plant cells.
To understand the basis of cell culture, and the changes imposed on cells under culture conditions.
To understand the techniques of fluorescence and electron microscopy and cell culture.

4. Animal Cell

5. Plant Cell

6. The Exercise Part I: Plant cells: Onion epidermis
Basic cell structure of a plant lacking chloroplasts
Iodine: observe nucleolus
Janus Green: observe mitochondria

7. The Exercise Part II: Animal cells: Human squamous epithelium
Observe differences from the plant cells
Do the mitochondria vary in size from those found in plant cells?

8. The Exercise Part III: Autofluorescence
View Tradescantia under the fluorescent microscope
Chlorophyll pigments autofluoresce

9. The Exercise Part IV: Anacharis Leaf Cells
View a living plant cell with chloroplasts

10. The Exercise Part V: Electron Micrographs and Cellular Infrastructure
View EM transparencies
Identify organelles
You may want to take notes for future identification (i.e. Exam 2 and Final)

11. Rough Endoplasmic Reticulum
Be Able to Identify:
Nucleus
Nuclear envelope
Nuclear pore
Nucleolus
Rough Endoplasmic Reticulum
Ribosome
Mitochondria

12. Be Able to Identify:
Golgi complex
top view
section view

13. Golgi
top view

14. Golgi
section view

15. Be Able to Identify:
Mitochondrion

16. Be Able to Identify:
Nucleus
Vacuole
Chloroplast

17. Be Able to Identify:
Thylakoid

18. Summary of Exercise 3 Part I: Onion epidermis Part II: Cheek cells
slide x 3: water / Iodine / Janus Green
size, nucleus, vacuole, cell wall/membrane, chloroplast, mitochondria (?)
Part II: Cheek cells
slide x 2: water / Janus Green
size, nucleus, cell membrane, mitochondria (?)
Part III: Tradescantia TA
Autofluorescence
Part IV: Anacharis
Cytoplasmic streaming