1. Degradable Chitosan-Collagen Composites Seeded with Cells as Tissue Engineered Heart Valves 
Jian-Hua Fu, PhD, Man Zhao, MD, Yan-Rong Lin, BS, Xu-Dong Tian, MD, Ya-Dong Wang, MD, Zhen-Xing Wang, MD, Le-Xin Wang, FRACGP, FCSANZ 
Heart, Lung and Circulation 
Volume 26, Issue 1, Pages (January 2017)
DOI: /j.hlc
Copyright © 2016 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ) Terms and Conditions

2. Figure 1
Aortic smooth muscle cell. A. HE staining: after smooth muscle cell cultivation in the fourth week, microscope shows cells with long spindle shape, cytoplasm pink, nucleus purple blue. HE×400; B. α-Actin histoimmunochemistry staining; α-Actin histoimmunochemistry staining presenting cytoplasm dye, there are brown fibre filaments paralleling with cell long axis, namely α-Actin filaments of smooth muscle. SABC×400.
Heart, Lung and Circulation  , DOI: ( /j.hlc )
Copyright © 2016 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ) Terms and Conditions

3. Figure 2
Bone marrow stromal stem cells – primary cells. Bone marrow stromal stem cells were cultured for third day, microscopically most of adherent cells elongate presenting shuttle-like.×40.
Heart, Lung and Circulation  , DOI: ( /j.hlc )
Copyright © 2016 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ) Terms and Conditions

4. Figure 3
Endothelial cells under microscope. The second generation of bone marrow stromal stem cells added into ECGS 22nd days. Under the microscope the cells are endothelial cells like.×40.
Heart, Lung and Circulation  , DOI: ( /j.hlc )
Copyright © 2016 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ) Terms and Conditions

5. Figure 4
Endothelial cells. A. HE staining: Endothelial cells induced and differentiated directionally from BMSCs seen under the microscope that the cells were polygonal, cytoplasm and nucleus is stained red, blue. HE×100; B. factor VIII immunohistochemistry staining: Immunohistochemistry staining of factor VIII of EC induced and differentiated from BMSC shows positive cells presenting polygon or short shuttle-like, cytoplasm abundance, containing brownish yellow granules, nucleus blue-stained and in middle of cell, cells arrangement filed in the same under microscope. SABC×200.
Heart, Lung and Circulation  , DOI: ( /j.hlc )
Copyright © 2016 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ) Terms and Conditions

6. Figure 5
Endothelial cells factor. A. Indirect immunofluorescence assay: Endothelial factor VIII immunofluorescence staining, positive cells were polygonal or short spindle under the microscope, abundant cytoplasm, with brown granules, nuclear black-green, in centre of cells, cells arranged as filed connected.×400; B. TEM examination: The transmission electron microscope shows the visible rod-shaped W-P bodies of endothelial cells, which are induced and differentiated from bone marrow stromal stem cells (shown as the white arrow). TEM×60000.
Heart, Lung and Circulation  , DOI: ( /j.hlc )
Copyright © 2016 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ) Terms and Conditions

7. Figure 6
Collagen-chitosan composite scaffold under SEM. A. The smooth muscle cells seeded on collagen- chitosan composite scaffold: Scanning electron microscope showed the smooth muscle cells seeded on collagen-chitosan composite scaffold, and a large number of cells in internal materials, with dense disordered arrangement. SEM×100; B. The smooth muscle cells, fibroblasts and endothelial cells seeded on collagen-chitosan composite scaffold: Scanning electron microscope shows the smooth muscle cells, fibroblasts and endothelial cells in turn seeded on collagen-chitosan composite material, and a large number of endothelial cells interspersed in long shuttle shape cell. SEM×900.
Heart, Lung and Circulation  , DOI: ( /j.hlc )
Copyright © 2016 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ) Terms and Conditions

8. Figure 7
Collagen-chitosan composite scaffold. A. HE staining: Collagen-chitosan composite scaffold presents a long-short mesh structure. HE×200; B. Collagen-chitosan composite material SEM tangential view: SEM shows a collagen-chitosan composite scaffold has regular pipeline with vertical and horizontal connection in the tangential view. SEM×80.
Heart, Lung and Circulation  , DOI: ( /j.hlc )
Copyright © 2016 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ) Terms and Conditions