1. Programmable Dielectrophoretic Chip for Cell Manipulation
Japaness Journal of Applied Physics 50 (2011) 06GL11
Programmable Dielectrophoretic Chip for Cell Manipulation
C. H. Chuang, Y.W .Huang, Y. T. Wu, and T. F. Wu
班級:碩機一甲
姓名:蘇琬婷
學號:MA110109

2. Outline Experimental Methods Results and discussion Conclusions
Simulation model
Design and fabrication of DEP chip
Experimental setup
Results and discussion
Simulation results
Cell manipulations
Conclusions

3. Simulation model
Fig. 1. (Color online) Cross-sectional schematic illustration of two different simulation models: (a) DEP chip with multilayer electrodes and microcavity array; (b) focusing electrode in the DEP chip.

4. Design and fabrication of DEP chip
Fig. 2. Microfabrication processes of programmable DEP chip:
(a) bottom electrode layer; (b) microcavity array; (c) focusing and middle electrode layer;(d) top electrode layer and chip bonding.

5. Experimental setup
Fig. 3. (Color online) Experimental setup for DEP focusing, trapping, and release of cells, including a LabVIEW program, a signal transmitting circuit,
and a DEP chip with focusing part and microcavity array part.

6. Outline Experimental Methods Results and discussion Conclusions
Simulation model
Design and frabrication of DEP chip
Experimental setup
Results and discussion
Simulation results
Cell manipulations
Conclusions

7. Simulation results
Fig. 4. (Color online) Simulation results of different types of focusing electrodes:
(a) triangle type, (b) fishbone type, and (c) taper type.
The profiles of theelectric field intensity of the three different focusing electrodes along the A–A0 cross section of the electrode surface where the gap between two electrodes
was the same at 20 μm.

8. Simulation results n-DEP n-DEP
Fig. 5. (Color online) Simulation results of DEP chip with multilayer electrodes and microcavity. (a) Contour of electric field as the AC signal was appliedto the middle and top electrodes.
(b) As AC signal was applied to the bottom and top electrodes.

9. Cell manipulations 10Vpp,50KHz 10Vpp,20KHz
Fig. 6. (Color online) Optical micrographs showing the focusing effect for bladder cancer cells. (a) Cells are suspended in the flow chamber near the focusing electrode
(b) The gap between the two electrodes is gradually reduced to gain the focusing effect, as
indicated in the red-circled area.
(c) Cells are focused within a width of 50μm and then flow toward the microcavity array.

10. Cell manipulations 10Vpp,80KHz 10Vpp,80KHz
Fig. 7. (Color online) Optical micrographs demonstrating the trapping
and programmable release of single cells:
(a) cells suspended in the microcavity array; (b) trapping of cells in the microcavity array; (c) and (d) two target cells, marked by red and blue circles, individually
released by programmable control.

11. Conclusions
Developed a novel dielectrophoresis (DEP) chip with multilayer electrodes for the focusing, trapping, and programmable release of bladder cells under a singlecell level.
A taper-type electrode provided the highest electric field and the best focusing effect for DEP manipulation.
The trapping and release of single cells was also easily achieved in a programmable control system.
This DEP chip has been proven to have great potential in cell examination and cell recovery for the diagnosis of cancer cells.

12. Thanks for your attension !!

13. 文獻參考:
Programmable Dielectrophoretic Chip for Cell Manipulations,
Cheng-Hsin Chuang, Yao-Wei Huang, Yao-Tung Wu, and Ting-Feng Wu
DOI: /JJAP.50.06GL11