1. Neurons, Synapses, and Signaling
Chapter 48
Neurons, Synapses, and Signaling

2. Overview: Lines of Communication
The cone snail kills prey with venom that disables neurons
__________________are nerve cells that transfer information within the body
Neurons use two types of signals to communicate: _____________(long-distance) and ___________________ (short-distance)
© 2011 Pearson Education, Inc.

3. Figure 48.1
Figure 48.1 What makes this snail such a deadly predator? 3

4. Interpreting signals in the nervous system involves sorting a complex set of ____________ _____________________
Processing of information takes place in simple clusters of neurons called ____________ or a more complex organization of neurons called a ____________
© 2011 Pearson Education, Inc.

5. Concept 48.1: Neuron organization and structure reflect function in information transfer
The __________ possesses extremely large nerve cells and has played a crucial role in the discovery of how neurons transmit signals
© 2011 Pearson Education, Inc.

6. Introduction to Information Processing
Nervous systems process information in three stages:_______________________________________________________
© 2011 Pearson Education, Inc.

7. Nerves with giant axons Ganglia
Figure 48.2
Nerves with giant axons
Ganglia
Brain
Arm
Eye
Mantle
Nerve
Figure 48.2 Overview of the squid nervous system. 7

8. Sensors detect external stimuli and internal conditions and transmit information along ______________________
Sensory information is sent to the brain or ganglia, where _______________ integrate the information
Motor output leaves the brain or ganglia via ___________________ , which trigger muscle or gland activity
© 2011 Pearson Education, Inc.

9. Many animals have a complex nervous system that consists of
A ______________________________where integration takes place; this includes the brain and a nerve cord
A _______________________________, which carries information into and out of the CNS
The neurons of the PNS, when bundled together, form ____________
© 2011 Pearson Education, Inc.

10. Peripheral nervous system (PNS) Central nervous system (CNS)
Figure 48.3
Sensory input
Integration
Sensor
Motor output
Figure 48.3 Summary of information processing.
Effector
Peripheral nervous system (PNS)
Central nervous system (CNS) 10

11. Neuron Structure and Function
Most of a neuron’s organelles are in the ______ _____________
Most neurons have ______________, highly branched extensions that receive signals from other neurons
The ________ is typically a much longer extension that transmits signals to other cells at _____________
The cone-shaped base of an axon is called the ________________
© 2011 Pearson Education, Inc.

12. Dendrites Stimulus Axon hillock Nucleus Cell body Presynaptic cell
Figure 48.4
Dendrites
Stimulus
Axon hillock
Nucleus
Cell body
Presynaptic cell
Axon
Signal direction
Synapse
Synaptic terminals
Figure 48.4 Neuron structure and organization.
Synaptic terminals
Postsynaptic cell
Neurotransmitter 12

13. A ________________ is a junction between an axon and another cell
The __________________ of one axon passes information across the synapse in the form of chemical messengers called _______________
A ________________ is a junction between an axon and another cell
© 2011 Pearson Education, Inc.

14. Most neurons are nourished or insulated by cells called ____________
Information is transmitted from a ____________ _______________ (a neuron) to a __________ _____________ (a neuron, muscle, or gland cell)
Most neurons are nourished or insulated by cells called ____________
© 2011 Pearson Education, Inc.

15. Dendrites Axon Cell body Portion of axon Sensory neuron Interneurons
Figure 48.5
Dendrites
Axon
Cell body
Figure 48.5 Structural diversity of neurons.
Portion of axon
Sensory neuron
Interneurons
Motor neuron 15

16. 80 m Glia Cell bodies of neurons Figure 48.6
Figure 48.6 Glia in the mammalian brain.
Cell bodies of neurons 16

17. Concept 48.2: Ion pumps and ion channels establish the resting potential of a neuron
Every cell has a voltage (difference in electrical charge) across its plasma membrane called a ________________________
The ________________ is the membrane potential of a neuron not sending signals
Changes in __________________ act as signals, transmitting and processing information
© 2011 Pearson Education, Inc.

18. Formation of the Resting Potential
In a mammalian neuron at resting potential, the concentration of ___ is highest inside the cell, while the concentration of ____ is highest outside the cell
_______________________ use the energy of ATP to maintain these K+ and Na+ gradients across the plasma membrane
These concentration gradients represent ______________________
© 2011 Pearson Education, Inc.

19. The opening of _______________in the plasma membrane converts ______________ potential to ______________ potential
A neuron at resting potential contains many open ____ channels and fewer open ___channels; ___ diffuses out of the cell
The resulting buildup of ____________________ within the neuron is the major source of membrane potential
© 2011 Pearson Education, Inc.

20. Table 48.1
Table 48.1 Ion Concentrations Inside and Outside of Mammalian Neurons 20

21. Sodium- potassium pump
Figure 48.7
Key
Na K
Sodium- potassium pump
OUTSIDE OF CELL
Potassium channel
Sodium channel
Figure 48.7 The basis of the membrane potential.
INSIDE OF CELL 21

22. Modeling the Resting Potential
Resting potential can be modeled by an artificial membrane that separates two chambers
The concentration of KCl is higher in the ______ chamber and lower in the outer chamber
____ diffuses down its gradient to the outer chamber
Negative charge (___) builds up in the inner chamber
At equilibrium, both the electrical and chemical gradients are balanced
© 2011 Pearson Education, Inc.

23. 140 mM KCl Inner chamber 90 mV Outer chamber 5 mM KCl K Cl
Figure 48.8a
Inner chamber
90 mV
Outer chamber
140 mM KCl
5 mM KCl K
Cl
Potassium channel
Artificial membrane
Figure 48.8 Modeling a mammalian neuron.
(a)
Membrane selectively permeable to K
EK  62 mV
 90 mV 23

24. Eion = 62 mV (log[ion]outside/[ion]inside)
The _____________________is the membrane voltage for a particular ion at equilibrium and can be calculated using the __________ equation
Eion = 62 mV (log[ion]outside/[ion]inside)
The equilibrium potential of K+ (EK) is ________, while the equilibrium potential of Na+ (ENa) is _____________
© 2011 Pearson Education, Inc.

25. In a resting neuron, the currents of K+ and Na+ are ________________, and the resting potential across the membrane remains steady
© 2011 Pearson Education, Inc.

26. 150 mM NaCl Inner chamber 62 mV Outer chamber 15 mM NaCl Cl Na
Figure 48.8b
Inner chamber
62 mV
Outer chamber
15 mM NaCl
150 mM NaCl
Cl
Na
Sodium channel
Figure 48.8 Modeling a mammalian neuron.
(b)
Membrane selectively permeable to Na
ENa  62 mV
 62 mV 26

27. Concept 48.3: Action potentials are the signals conducted by axons
Changes in membrane potential occur because neurons contain __________________ that open or close in response to stimuli
© 2011 Pearson Education, Inc.

28. Hyperpolarization and Depolarization
When gated ________________ open, K+ diffuses________, making the inside of the cell more negative
This is__________________, an increase in magnitude of the membrane potential
© 2011 Pearson Education, Inc.

29. Membrane potential (mV)
Figure 48.10a
(a)
Graded hyperpolarizations produced by two stimuli that increase membrane permeability to K
Stimulus
50
Membrane potential (mV)
Threshold
50
Figure Graded potentials and an action potential in a neuron.
Resting potential
Hyperpolarizations
100 1 2 3 4 5
Time (msec) 29

30. Opening other types of ion channels triggers a _________________, a reduction in the magnitude of the membrane potential
For example, depolarization occurs if ________ ___________________ and Na+ diffuses into the cell
© 2011 Pearson Education, Inc.

31. Membrane potential (mV)
Figure 48.10b
(b)
Graded depolarizations produced by two stimuli that increase membrane permeability to Na
Stimulus
50
Membrane potential (mV)
Threshold
50
Figure Graded potentials and an action potential in a neuron.
Resting potential
Depolarizations
100 1 2 3 4 5
Time (msec) 31

32. Graded Potentials and Action Potentials
___________________ are changes in polarization where the magnitude of the change varies with the strength of the stimulus
These are not the nerve signals that travel along axons, but they do have an effect on the generation of nerve signals
© 2011 Pearson Education, Inc.

33. If a depolarization shifts the membrane potential sufficiently, it results in a massive change in membrane voltage called an ________________
Action potentials have a constant magnitude, are ____________, and transmit signals over long distances
They arise because some ion channels are ____________________, opening or closing when the membrane potential passes a certain level
© 2011 Pearson Education, Inc.

34. Strong depolarizing stimulus
Figure 48.10c
(c)
Action potential triggered by a depolarization that reaches the threshold
Strong depolarizing stimulus
50
Action potential
Membrane potential (mV)
Threshold
50
Figure Graded potentials and an action potential in a neuron.
Resting potential
100 1 2 3 4 5 6
Time (msec) 34

35. Generation of Action Potentials: A Closer Look
An action potential can be considered as a series of stages
At ___________________
Most voltage-gated sodium (Na+) channels are _____________; most of the voltage-gated potassium (K+) channels are also __________
© 2011 Pearson Education, Inc.

36. Membrane potential (mV)
Figure
Key
Na K
50
Membrane potential (mV)
Threshold
50 1
Resting potential
100
Figure The role of voltage-gated ion channels in the generation of an action potential.
Time
OUTSIDE OF CELL
Sodium channel
Potassium channel
INSIDE OF CELL
Inactivation loop 1
Resting state 36

37. When an _________________ is generated
Voltage-gated Na+ channels ________ and Na+ flows __________________
During the ________, the threshold is crossed, and the membrane potential _____________
During the ____________, voltage-gated Na+ channels become _____________; voltage-gated K+ channels _________, and K+ flows _________________
© 2011 Pearson Education, Inc.

38. 5. During the _____________, membrane permeability to _____is at first higher than at rest, then voltage-gated K+ channels close and ___________________ is restored
© 2011 Pearson Education, Inc.

39. Falling phase of the action potential 3
Figure
Key
Na K 4
Falling phase of the action potential 3
Rising phase of the action potential
50
Action potential 3
Membrane potential (mV)
Threshold 4 2
50 1 1 5 2
Depolarization
Resting potential
100
Figure The role of voltage-gated ion channels in the generation of an action potential.
Time
OUTSIDE OF CELL
Sodium channel
Potassium channel
INSIDE OF CELL
Inactivation loop 1
Resting state 5
Undershoot 39

40. Membrane potential (mV)
Figure 48.11a
50
Action potential 3
Membrane potential (mV) 2 4
Threshold
50 1
Figure The role of voltage-gated ion channels in the generation of an action potential. 1 5
Resting potential
100
Time 40

41. During the ________________ after an action potential, a second action potential cannot be initiated
The refractory period is a result of a temporary inactivation of the __________________
© 2011 Pearson Education, Inc.

42. Conduction of Action Potentials
At the site where the action potential is generated, usually the axon hillock, an electrical current _________________ the neighboring region of the axon membrane
Action potentials travel in only one direction: toward the ____________________
© 2011 Pearson Education, Inc.

43. _______________________ behind the zone of depolarization prevent the action potential from traveling backwards
© 2011 Pearson Education, Inc.

44. Axon Plasma membrane Action potential 1 Cytosol Action potential 2
Figure
Axon
Plasma membrane
Action potential 1
Cytosol
Na
Action potential K 2
Na
Figure Conduction of an action potential. K
Action potential K 3
Na K 44

45. Evolutionary Adaptation of Axon Structure
The speed of an action potential increases with the ___________________
In vertebrates, axons are insulated by a ______ ________________, which causes an action potential’s speed to increase
Myelin sheaths are made by glia— ____________________in the CNS and _____________________in the PNS
© 2011 Pearson Education, Inc.

46. Nucleus of Schwann cell Axon Myelin sheath
Figure 48.13
Node of Ranvier
Layers of myelin
Axon
Schwann cell
Schwann cell
Nodes of Ranvier
Nucleus of Schwann cell
Axon
Myelin sheath
Figure Schwann cells and the myelin sheath.
0.1 m 46

47. Action potentials are formed only at ________ _______________, gaps in the myelin sheath where voltage-gated Na+ channels are found
Action potentials in myelinated axons jump between the nodes of Ranvier in a process called _______________________
© 2011 Pearson Education, Inc.

48. Depolarized region (node of Ranvier)
Figure 48.14
Schwann cell
Depolarized region (node of Ranvier)
Cell body
Myelin sheath
Axon
Figure Saltatory conduction. 48

49. Concept 48.4: Neurons communicate with other cells at synapses
At __________________, the electrical current flows from one neuron to another
At __________________, a chemical neurotransmitter carries information across the gap junction
Most synapses are ______________synapses
© 2011 Pearson Education, Inc.

50. The action potential causes the release of the ___________________
The presynaptic neuron synthesizes and packages the neurotransmitter in _________ _____________located in the synaptic terminal
The action potential causes the release of the ___________________
The neurotransmitter diffuses across the ____________________and is received by the postsynaptic cell
© 2011 Pearson Education, Inc.

51. Presynaptic cell Synaptic cleft
Figure 48.15
Presynaptic cell
Postsynaptic cell
Axon
Synaptic vesicle containing neurotransmitter 1
Postsynaptic membrane
Synaptic cleft
Presynaptic membrane 3
Figure A chemical synapse. K
Ca2 2
Voltage-gated Ca2 channel
Ligand-gated ion channels 4
Na 51

52. Generation of Postsynaptic Potentials
Direct synaptic transmission involves binding of neurotransmitters to _____________________ in the postsynaptic cell
Neurotransmitter binding causes ion channels to open, generating a _______________________
© 2011 Pearson Education, Inc.

53. Postsynaptic potentials fall into two categories
____________________________________ are depolarizations that bring the membrane potential toward threshold
____________________________________ are hyperpolarizations that move the membrane potential farther from threshold
© 2011 Pearson Education, Inc.

54. After release, the neurotransmitter
May diffuse out of the __________________
May be ___________ by surrounding cells
May be degraded by _________________
© 2011 Pearson Education, Inc.

55. Summation of Postsynaptic Potentials
Most neurons have ______________on their dendrites and cell body
A single EPSP is usually ________________ to trigger an action potential in a postsynaptic neuron
© 2011 Pearson Education, Inc.

56. If two EPSPs are produced in rapid succession, an effect called ____________________occurs
© 2011 Pearson Education, Inc.

57. Terminal branch of presynaptic neuron
Figure 48.17a
Terminal branch of presynaptic neuron E1 E1 E2 E2
Axon hillock
Postsynaptic neuron I I
Threshold of axon of postsynaptic neuron
Action potential
Membrane potential (mV)
Figure Summation of postsynaptic potentials.
Resting potential
70 E1 E1 E1 E1
Subthreshold, no summation
(a)
(b) Temporal summation 57

58. In________________, EPSPs produced nearly simultaneously by different synapses on the same postsynaptic neuron add together
The combination of EPSPs through ________ and _______________ summation can trigger an action potential
© 2011 Pearson Education, Inc.

59. Spatial summation of EPSP and IPSP (d)
Figure 48.17b E1 E1 E2 E2 I I
Action potential
Figure Summation of postsynaptic potentials.
E1  E2 E1 I
E1  I
(c) Spatial summation
Spatial summation of EPSP and IPSP
(d) 59

60. Through summation, an IPSP can ___________ the effect of an EPSP
The ________________ of EPSPs and IPSPs determines whether an axon hillock will reach threshold and generate an action potential
© 2011 Pearson Education, Inc.
© 2011 Pearson Education, Inc.

61. Modulated Signaling at Synapses
In some synapses, a neurotransmitter binds to a receptor that is ___________________
In this case, movement of ions through a channel depends on one or more ___________ _________________
© 2011 Pearson Education, Inc.

62. Binding of a neurotransmitter to a metabotropic receptor activates a signal transduction pathway in the postsynaptic cell involving a ___________ _________________
Compared to ligand-gated channels, the effects of second-messenger systems have a _________________ but _______________
© 2011 Pearson Education, Inc.

63. Neurotransmitters
There are more than ______________________, belonging to five groups: acetylcholine, biogenic amines, amino acids, neuropeptides, and gases
A single neurotransmitter may have more than a ____________ different receptors
© 2011 Pearson Education, Inc.

64. Table 48.2
Table 48.2 Major Neurotransmitters 64

65. Acetylcholine
____________________ is a common neurotransmitter in vertebrates and invertebrates
It is involved in muscle stimulation, memory formation, and learning
Vertebrates have two major classes of acetylcholine receptor, one that is ___________ and one that is _________________
© 2011 Pearson Education, Inc.

66. Amino Acids ____________________________are active in the CNS and PNS
Known to function in the CNS are
______________________________
_______________
© 2011 Pearson Education, Inc.

67. Biogenic Amines _____________________include
They are active in the CNS and PNS
© 2011 Pearson Education, Inc.

68. Neuropeptides
Several ________________, relatively short chains of amino acids, also function as neurotransmitters
Neuropeptides include substance P and _________________, which both affect our perception of pain
________________ bind to the same receptors as endorphins and can be used as painkillers
© 2011 Pearson Education, Inc.

69. Concentration That Blocked Naloxone Binding
Figure 48.18
EXPERIMENT
Radioactive naloxone 1
Radioactive naloxone and a test drug are incubated with a protein mixture.
Drug
Protein mixture 2
Proteins are trapped on a filter. Bound naloxone is detected by measuring radioactivity.
RESULTS
Concentration That Blocked Naloxone Binding
Figure Inquiry: Does the brain have a specific protein receptor for opiates?
Drug
Opiate
Morphine
Yes
6  109 M
Methadone
Yes
2  108 M
Levorphanol
Yes
2  109 M
Phenobarbital No
No effect at 104 M
Atropine No
No effect at 104 M
Serotonin No
No effect at 104 M 69

70. EXPERIMENT Radioactive naloxone
Figure 48.18a
EXPERIMENT
Radioactive naloxone 1
Radioactive naloxone and a test drug are incubated with a protein mixture.
Drug
Protein mixture
Figure Inquiry: Does the brain have a specific protein receptor for opiates? 2
Proteins are trapped on a filter. Bound naloxone is detected by measuring radioactivity. 70

71. Concentration That Blocked Naloxone Binding
Figure 48.18b
RESULTS
Concentration That Blocked Naloxone Binding
Drug
Opiate
Morphine
Yes
6  109 M
Methadone
Yes
2  108 M
Levorphanol
Yes
2  109 M
Phenobarbital No
No effect at 104 M
Figure Inquiry: Does the brain have a specific protein receptor for opiates?
Atropine No
No effect at 104 M
Serotonin No
No effect at 104 M 71

72. Gases
Gases such as ________________and ______ _____________are local regulators in the PNS
© 2011 Pearson Education, Inc.