1. Figure Legend: From: B-RAF signaling promotes optic nerve regeneration
Invest. Ophthalmol. Vis. Sci ;55(13):1439.
Figure Legend:
Activation of B-RAF enables regenerative axon growth in the crush-lesioned optic nerve via the canonical effectors MEK1/MEK2. (A) Experimental time course. (B) Intravitreal injection of AAV-Cre induces expression of TdTomato reporter in RGCs. (C) Upper, whole mount crushed Bax -/- optic nerve. Crush site is indicated by a red asterisk. Bax null background was used to prevent RGC death after optic nerve crush. Lower, confocal fluorescence image of the same nerve. Green, axons anterogradely labeled with CTB-Alexa488. (D) Regenerative growth in lesioned kaB-RAF expressing optic nerve. Inset, axons at approx. 3.5 mm from the crush site. (E) Loss of MEK1 and MEK2 abolishes the regeneration driven by kaB-RAF. (F) Optic nerve regeneration in absence of PTEN. (G) Quantification. Scale bar = 0.5 mm. N=3 for each group.
Date of download: 10/6/2017
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