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Published byKatrina Lamb Modified over 6 years ago
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From: Expression Profile of Nerve Growth Factor after Muscle Incision in the Rat
Anesthes. 2009;110(1): doi: /ALN.0b013e318190bc84 Figure Legend: Fig. 2. Nerve growth factor (NGF) expression in incised or sham-operated plantar flexor digitorum brevis muscle. ( A ) Changes in NGF messenger RNA (mRNA) relative to β-actin mRNA examined by real-time quantitative-polymerase chain reaction. Data are expressed as mean ± SEM of ratio of incised flexor muscle to sham controls. ( B ) NGF protein determined by enzyme-linked immunosorbent assay. Data are shown as mean ± SEM of nanogram of NGF protein per milligram of protein. ( C ) Western blots for NGF and actin immunoreactivity. Top: Example of Western blot showing immunoreactive NGF and actin control bands in detailed time course after plantar incision. Bottom: Summary of relative changes in NGF protein expression after plantar incision. Data are expressed as mean ± SEM of ratio of incised flexor muscle to sham controls. Non = contralateral nonincised muscle. PO = post operative. POD = postoperative day. * P < 0.05, ** P < 0.01 versus sham. N = 6 per group. Date of download: 10/5/2017 Copyright © 2017 American Society of Anesthesiologists. All rights reserved.
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