1. Bioluminescence imaging as a tool to evaluate germ cells in vitro and transplantation in vivo as fertility preservation of prepubertal male mice 
Chi-Huang Chen, M.D., Chia-Woei Wang, M.D., Ming-I. Hsu, M.D., Yen-Hua Huang, Ph.D., Wen-Fu Thomas Lai, D.M.D., D.M.Sc., Chii- Ruey Tzeng, M.D. 
Fertility and Sterility 
Volume 97, Issue 5, Pages (May 2012)
DOI: /j.fertnstert
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1 Scheme of the study design.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
(A1–A3) Microinjection of a seminiferous tubule with spermatogonial stem cells under a dissecting microscope using a hand-pulled glass needle attached to a mouth micropipette. The cells flow to the other tubules through the rete testis, to which all tubules are connected. Tracking their passage was facilitated by mixing diluted indigo carmine dye with the injection medium. Bar = 1 mm. Histology of the testis from a busulfan-treated immature wild-type mouse showing immature seminiferous tubules (B1), atrophic seminiferous tubules and spermatogenetic failure (B2), and normal spermatogenesis (B3). Bar = 100 μm.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Bioluminescence imaging using pseudocolor mapping for quantification, showing localized luminescence output from isolated spermatogonia per well: 0, 10, 102, 103, 104, 105, and 106 from left to right seeded in a 96-well plate (upper). Little variation was seen in three measurements of pseudocolor mapping graphed as a function of the number of spermatogonia per well: 0, 10, 102, 103, 104, 105, and 106 from left to right seeded in a 96-well plate. The bioluminescence imaging intensity was linearly proportional to the cell number per well (lower).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Testes of recipient wild-type mice after transplantation of germ cells from FVB/N-Tg (PolII-luc) Ltc transgenic mice, demonstrated by bioluminescence imaging in vivo (A, left). Control testis without transplantation of germ cells from FVB/N-Tg (PolII-luc) Ltc transgenic mice (A, right). Part of 3-day-old live pups (B) carrying the transgene also showed bioluminescence (C). This confirmed that the transgenic line had been maintained in the hemizygous offspring (C).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions