1. Supplementary Fig. 1
***
Cell viability (%)
HepG2 3
(hr)
DU145
A549 17 48 75 63
Cleaved
Caspase-3
β-actin
Hsp70
HS (43ºC)
MEF A B C
Mr (K)
Supplementary Fig. 1. (A) MEF were exposed to a 43 ºC heat stress for 3 hr. Lysates were blotted for cleaved caspase-3 and β-actin. (B) MEF were exposed to a 43 ºC heat stress for the indicated times and then incubated for 24 hr. Cell viability was then measured. Data represent the mean ± SEM. ***P<0.001 versus Control (ANOVA, Dunnett's test). (C) HepG2, DU145, or A549 cells were exposed to a 43 ºC heat stress for the indicated times. Lysates were blotted for cleaved caspase-3, Hsp70, and β-actin.

2. Supplementary Fig. 2
DMSO
Bip
IRE1α
130
100 75 63 17 11 48 35
DTT
β-actin
LC3-II
LC3-I
Mr (K) Tm Tg
Supplementary Fig. 2. HeLa cells were treated with DMSO, thapsigargin (Tg, 0.4 or 1.0 μM), tunicamycin (Tm, 2 or 10 μg/ml), or dithiothreitol (DTT, 0.5 mM) for 8 hr. Lysates were blotted for IRE1α, Bip, LC3, and β-actin.

3. Supplementary Fig. 3 A
100 75
135 63 20 25 35 48 17
IRE1α
(full-length) B
Mr (K) 3 6
HeLa
(hr)
43ºC 1
MEF *
Supplementary Fig. 3. (A) Uncropped images of an immunoblot for IRE1α in HeLa cells. (B) Uncropped images of an immunoblot for IRE1α in MEF. The asterisk represents a nonspecific band.